An Hsp70 promoter drives expression of a mutated form of Scm, in which the sequence for codons 174 to 379 have been substituted with an Ala-Ala-Gly linker.
Animals expressing ScmΔMBT.hs under a regime of 1 hour heat shocks at 37[o]C every 12 hours throughout larval and pupal development, starting 30 hours after egg laying do not develop beyond puparium formation.