FlyBase Allele Report: Dmel\NT1[RNAi.CK.UAS]

General Information

Symbol

Dmel\NT1^RNAi.CK.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0240746

Feature type

allele

Associated gene

Dmel\NT1

Associated Insertion(s)

Carried in Construct

P{UAS-NT1.CK.RNAi}

Key Links

Transgenic product class

RNAi_reagent

(Zhu et al., 2008)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Zhu et al., 2008)

Mutagen

in vitro construct

(Zhu et al., 2008)

Progenitor genotype

Carried in construct

P{UAS-NT1.CK.RNAi}

Cytology

Description

UAS regulatory sequences drive expression of inverted repeat sequences corresponding to the cystine-knot region of NT1.

(Zhu et al., 2008)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

NT1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with NT1⁴¹, Scer\GAL4^how-24B

(Zhu et al., 2008)

increased cell death | embryonic stage 17, with NT1⁴¹, Scer\GAL4^sim.PS

(Zhu et al., 2008)

Phenotype Manifest In

larval intersegmental nerve branch ISNb of A1-7, with NT1⁴¹, Scer\GAL4^how-24B

(Zhu et al., 2008)

larval intersegmental nerve branch ISNd of A1-7, with NT1⁴¹, Scer\GAL4^how-24B

(Zhu et al., 2008)

larval segmental nerve, with NT1⁴¹, Scer\GAL4^how-24B

(Zhu et al., 2008)

Detailed Description

Statement

Reference

Targeted expression of NT1^{dsRNA.CK.Scer\UAS} in the CNS midline (under the control of Scer\GAL4^sim.PS) in a NT1⁴¹ heterozygous background results in a significant increase in apoptosis throughout the CNS cortex compared to controls at embryonic stage 17.

Targeted expression of NT1^{dsRNA.CK.Scer\UAS} in the muscle (under the control of Scer\GAL4^how-24B) in a NT1⁴¹ heterozygous background results in a significant increase in the incidence of misrouting phenotypes in ISNb/d and SN fascicles compared to wild-type, including effects in more than one projection per hemisegment (e.g. misrouting plus loss).

Targeted expression of NT1^{IR.CK.Scer\UAS} in the CNS midline (under the control of Scer\GAL4^sim.PS) in a NT1^41.Δ.w heterozygous background results in a significant increase in apoptosis throughout the CNS cortex compared to controls at embryonic stage 17.

Targeted expression of NT1^{IR.CK.Scer\UAS} in the muscle (under the control of Scer\GAL4^how-24B) in a NT1^41.Δ.w heterozygous background results in a significant increase in the incidence of misrouting phenotypes in ISNb/d and SN fascicles compared to wild-type, including effects in more than one projection per hemisegment (e.g. misrouting plus loss).

(Zhu et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

NT1^{IR.CK.Scer\UAS}

NT1^RNAi.CK.UAS

NT1^{dsRNA.CK.Scer\UAS}

NT1^dsRNA.CK.UAS

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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