FlyBase Allele Report: Dmel\Shark[RNAi.UAS.WIZ]

General Information

Symbol

Dmel\Shark^RNAi.UAS.WIZ

Species

D. melanogaster

Name

FlyBase ID

FBal0240977

Feature type

allele

Associated gene

Dmel\Shark

Associated Insertion(s)

Carried in Construct

P{UAS-Shark.RNAi.WIZ}

Also Known As

UAS-shark^RNAi

Key Links

Transgenic product class

RNAi_reagent

(Ziegenfuss et al., 2008)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ziegenfuss et al., 2008)

Mutagen

in vitro construct

(Ziegenfuss et al., 2008)

Progenitor genotype

Carried in construct

P{UAS-Shark.RNAi.WIZ}

Cytology

Description

UAS regulatory sequences drive expression of an inverted repeat of a 522bp fragment corresponding to nucleotides 577-1098 of Shark cDNA LD41606.

(Ziegenfuss et al., 2008)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Shark

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates Huntington's disease

modeled by Hsap\HTT^{Q91.ex1.QUAS.mCherry}

(Pearce et al., 2015)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult ensheathing glial cell, with Scer\GAL4^Mz709

(Doherty et al., 2009)

adult ensheathing glial cell, with Scer\GAL4^repo.PU

(Doherty et al., 2009)

Detailed Description

Statement

Reference

Expression of shark^{dsRNA.Scer\UAS.WIZ} under the control of either Scer\GAL4^repo.PU or Scer\GAL4^Mz709 strongly suppresses the recruitment of ensheathing glia membranes to severed olfactory receptor neuron axons after severing of the maxillary palp. Glial clearance of axonal debris from the central nervous system is blocked in these animals. Expression of shark^{dsRNA.Scer\UAS.WIZ} under the control of Scer\GAL4^alrm.PD has no effect on axon debris clearance after severing of the maxillary palp.

(Doherty et al., 2009)

Glial membranes are not recruited to severed axons following ablation of maxillary palps in animals expressing shark^{dsRNA.Scer\UAS.WIZ} under the control of Scer\GAL4^repo.PU, in contrast to control animals. Clearance of severed olfactory receptor neuron axons following ablation of maxillary palps appears to be suppressed in animals expressing shark^{dsRNA.Scer\UAS.WIZ} under the control of Scer\GAL4^repo.PU.

Glial hypertrophy is also not seen after severing of antennal olfactory receptor neuron axons in animals expressing shark^{dsRNA.Scer\UAS.WIZ} under the control of Scer\GAL4^repo.PU, in contrast to control animals.

(Ziegenfuss et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

Shark^RNAi.UAS.WIZ

Shark^{dsRNA.Scer\UAS.WIZ}

Shark^{dsRNA.UAS.WIZ}

shark^RNAi

(Pearce et al., 2015)

shark^{dsRNA.Scer\UAS.WIZ}

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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