FlyBase Allele Report: Dmel\CTCF[P35.2]

General Information

Symbol

Dmel\CTCF^P35.2

Species

D. melanogaster

Name

FlyBase ID

FBal0241199

Feature type

allele

Associated gene

Dmel\CTCF

Associated Insertion(s)

Carried in Construct

Also Known As

CTCF^35.2

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Mohan et al., 2007)

Progenitor genotype

CTCF^GE22007

(Mohan et al., 2007)

Cytology

Description

Imprecise excision of P{EP}CTCF^GE22007 generates a deletion in CTCF.

(Mohan et al., 2007)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

abdominal segment 5

(Mohan et al., 2007)

abdominal segment 6 | pharate adult stage (with CTCF⁹)

(Savitsky et al., 2016)

abdominal segment 6 | pharate adult stage (with CTCF^y+1)

(Savitsky et al., 2016)

abdominal segment 7 | pharate adult stage (with CTCF⁹)

(Savitsky et al., 2016)

abdominal segment 7 | pharate adult stage (with CTCF^y+1)

(Savitsky et al., 2016)

abdominal segment 8 | pharate adult stage (with CTCF⁹)

(Savitsky et al., 2016)

abdominal segment 8 | pharate adult stage (with CTCF^y+1)

(Savitsky et al., 2016)

abdominal sternite 5

(Mohan et al., 2007)

abdominal sternite 6 & abdominal sternite bristle

(Mohan et al., 2007)

abdominal sternite | pharate adult stage (with CTCF⁹)

(Savitsky et al., 2016)

abdominal sternite | pharate adult stage (with CTCF^y+1)

(Savitsky et al., 2016)

abdominal sternite bristle | pharate adult stage (with CTCF⁹)

(Savitsky et al., 2016)

abdominal sternite bristle | pharate adult stage (with CTCF^y+1)

(Savitsky et al., 2016)

abdominal tergite 4

(Mohan et al., 2007)

abdominal tergite 5

(Mohan et al., 2007)

abdominal tergite 7

(Mohan et al., 2007)

abdominal tergite 7 & abdominal sternite bristle

(Mohan et al., 2007)

abdominal tergite | pharate adult stage (with CTCF⁹)

(Savitsky et al., 2016)

abdominal tergite | pharate adult stage (with CTCF^y+1)

(Savitsky et al., 2016)

male genitalia

(Mohan et al., 2007)

male genitalia | pharate adult stage (with CTCF⁹)

(Savitsky et al., 2016)

male genitalia | pharate adult stage (with CTCF^y+1)

(Savitsky et al., 2016)

Detailed Description

Statement

Reference

CTCF^P35.2/CTCF⁹ have pharate adult lethality, with rare escapers that eclose but die within 24-48hr, and show posterior-to-anterior homeotic transformations of abdominal segments A8-A6: in males genitalia is rotated by approximately 20-180[o] (90%), s6 (sternite of the sixth segment) has 4-8 (average 6) bristles; in females s7 has 12-16 (average 14) bristles, shape of s7 transformed toward s6 and bristles lose orientation, and t8 (tergite of the eighth segment) has rows of large bristles.

CTCF^y+1/CTCF^P35.2 males and females extracted from pupal cases show posterior-to-anterior homeotic transformations of abdominal segments A8-A6: in males genitalia is rotated 90-180[o] (100% penetrance) and s6 has 6-10 (average 8) bristles; in females s7 has 11-14 (average 12) bristles, the shape of s7 is transformed toward s6 and bristles lose orientation, and t8 has rows of large bristles.

(Savitsky et al., 2016)

CTCF^P35.2 mutant salivary glands display dramatic vesiculation of the nucleolar structure.

(Guerrero and Maggert, 2011)

CTCF^P35.2 homozygotes are pharate lethal. Male genitalia are rotated by 40[o]-90[o] in 100% of cases. Males exhibit A4 tergites with patchy pigmentation. A5 tergites show a few white spots, the A5 sternite is elongated into a banana shape. Eight bristles are found on the sixth sternite, and the seventh tergite is smaller than in wild-type. CTCF^P35.2 homozygous females exhibit A7 sternites with up to 11 bristles pointing vertically downwards.

(Mohan et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

CTCF^P35.2/CTCF^y+1 is rescued by CTCF^{Ubi-p63E.Tag:StrepII}

(Savitsky et al., 2016)

Comments

CTCF^{Ubi-p63E.T:Zzzz\StrepII} rescues viability of CTCF^y+1/CTCF^P35.2 flies.

(Savitsky et al., 2016)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

CTCF^35.2

(Savitsky et al., 2016, Guerrero and Maggert, 2011)

CTCF^P35.2

(Savitsky et al., 2016, Mohan et al., 2007)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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