FlyBase Allele Report: Dmel\Axud1[RNAi.UAS]

General Information

Symbol

Dmel\Axud1^RNAi.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0241585

Feature type

allele

Associated gene

Dmel\Axud1

Associated Insertion(s)

Carried in Construct

P{UAS-Axud1.RNAi}

Key Links

Transgenic product class

RNAi_reagent

(Glavic et al., 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Glavic et al., 2009)

Mutagen

in vitro construct

(Glavic et al., 2009)

Progenitor genotype

Carried in construct

P{UAS-Axud1.RNAi}

Cytology

Description

UAS regulatory sequences drive expression of an inverted repeat of a 473 bp fragment corresponding to the coding region of Axud1.

(Glavic et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Axud1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell growth, with Scer\GAL4^hh-Gal4

(Glavic et al., 2009)

abnormal mitotic cell cycle | larval stage, with Scer\GAL4^hh-Gal4

(Glavic et al., 2009)

abnormal size, with Scer\GAL4^hh-Gal4

(Glavic et al., 2009)

decreased cell size, with Scer\GAL4^hh-Gal4

(Glavic et al., 2009)

visible, with Scer\GAL4^hh-Gal4

(Glavic et al., 2009)

Phenotype Manifest In

cell & wing blade, with Scer\GAL4^hh-Gal4

(Glavic et al., 2009)

wing blade, with Scer\GAL4^hh-Gal4

(Glavic et al., 2009)

Detailed Description

Statement

Reference

Scer\GAL4^hh-Gal4-driven expression of Axud1^{dsRNA.Scer\UAS} in the posterior compartment of the larval wing imaginal disc engenders a smaller than normal wing size, without noticeable effects on wing patterning or cell differentiation.

Wing cells expressing Axud1^{dsRNA.Scer\UAS} under the control of Scer\GAL4^hh-Gal4, are on average 24% smaller than wild-type cells. The reduction in cell size accounts for the smaller posterior compartment size in Scer\GAL4^hh-Gal4-driven Axud1^{dsRNA.Scer\UAS}-expressing wings.

The fraction of cells in the S phase of the cell cycle is higher in cells expressing Scer\GAL4^hh-Gal4-driven Axud1^{dsRNA.Scer\UAS} compared to wild-type cells. However, the frequency of mitotic cells is not significantly modified in Axud1^{dsRNA.Scer\UAS}-expressing third instar larval wing imaginal discs (under the control of Scer\GAL4^hh-Gal4). The final number of cells appears normal and cell death is not induced as a result of Scer\GAL4^hh-Gal4-driven Axud1^{dsRNA.Scer\UAS} expression.

Third instar wing imaginal disc cells expressing Scer\GAL4^hh-Gal4-driven Axud1^{dsRNA.Scer\UAS} possess clonal growth advantage compared to surrounding cells expressing higher levels of Axud1.

(Glavic et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Axud1^RNAi.UAS, Scer\GAL4^hh-Gal4 has decreased cell size phenotype, enhanceable by Dcr-2^UAS.cDa, Scer\GAL4^hh-Gal4

(Glavic et al., 2009)

Axud1^RNAi.UAS, Scer\GAL4^hh-Gal4 has abnormal size phenotype, enhanceable by Dcr-2^UAS.cDa, Scer\GAL4^hh-Gal4

(Glavic et al., 2009)

NOT Enhanced by

Statement

Reference

Axud1^RNAi.UAS, Scer\GAL4^hh-Gal4 has abnormal size phenotype, non-enhanceable by Dronc^C318G.UAS, Scer\GAL4^hh-Gal4

(Glavic et al., 2009)

NOT suppressed by

Statement

Reference

Axud1^RNAi.UAS, Scer\GAL4^hh-Gal4 has abnormal size phenotype, non-suppressible by Dronc^C318G.UAS, Scer\GAL4^hh-Gal4

(Glavic et al., 2009)

Phenotype Manifest In

Enhanced by

Statement

Reference

Axud1^RNAi.UAS, Scer\GAL4^hh-Gal4 has cell & wing blade phenotype, enhanceable by Dcr-2^UAS.cDa, Scer\GAL4^hh-Gal4

(Glavic et al., 2009)

Axud1^RNAi.UAS, Scer\GAL4^hh-Gal4 has wing blade phenotype, enhanceable by Dcr-2^UAS.cDa, Scer\GAL4^hh-Gal4

(Glavic et al., 2009)

NOT Enhanced by

Statement

Reference

Axud1^RNAi.UAS, Scer\GAL4^hh-Gal4 has wing blade phenotype, non-enhanceable by Dronc^C318G.UAS, Scer\GAL4^hh-Gal4

(Glavic et al., 2009)

NOT suppressed by

Statement

Reference

Axud1^RNAi.UAS, Scer\GAL4^hh-Gal4 has wing blade phenotype, non-suppressible by Dronc^C318G.UAS, Scer\GAL4^hh-Gal4

(Glavic et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

The cell size reduction resulting from the Scer\GAL4^hh-Gal4-driven expression of Axud1^{dsRNA.Scer\UAS} is increased to 31% in wings co-expressing Dcr-2^Scer\UAS.cDa.

The wing size reduction resulting from the Scer\GAL4^hh-Gal4-driven expression of Axud1^{dsRNA.Scer\UAS} is enhanced by the co-expression of Dcr-2^Scer\UAS.cDa.

The wing size reduction resulting from the Scer\GAL4^hh-Gal4-driven expression of Axud1^{dsRNA.Scer\UAS} is not affected by the co-expression of Nc^{C318G.Scer\UAS}.

(Glavic et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^salm.EPv/Axud1^RNAi.UAS rescues Axud1^C676

(Glavic et al., 2009)

Comments

Scer\GAL4^salm-EPv-driven co-expression of Axud1^{dsRNA.Scer\UAS} with Axud1^C676 rescues the wing phenotypes associated with the ectopic expression of Axud1^C676.

(Glavic et al., 2009)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Expression of Axud1^{dsRNA.Scer\UAS} produces dsRNA which results in dsRNA interference (RNAi) of the Axud1 gene.

(Glavic et al., 2009)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Axud1^RNAi.UAS

Axud1^{dsRNA.Scer\UAS}

Axud1^dsRNA.UAS

Name Synonyms

Secondary FlyBase IDs

References (2)

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