FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\htt+m22.7
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General Information
Symbol
Dmel\htt+m22.7
Species
D. melanogaster
Name
FlyBase ID
FBal0241669
Feature type
allele
Associated gene
Dmel\htt
Associated Insertion(s)
Carried in Construct
P{htt+m22.7}
Key Links
Transgenic product class
wild_type
(Zhang et al., 2009)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Zhang et al., 2009)
Mutagen
in vitro construct
(Zhang et al., 2009)
Progenitor genotype
Carried in construct
P{htt+m22.7}
Cytology
Description

Full length htt is expressed under the control of its endogenous regulatory region. The 22.7kb minigene consists of a 14.9kb genomic fragment containing 5' regulatory sequences together with the first ten introns and exons, followed by a 6.6kb cDNA fragment that contains from exon 10 to exon 29, followed by a 1.2kb genomic fragment containing the exon 29 and the remaining polyA sites and untranscribed region at the 3' end of the gene.

(Zhang et al., 2009)
Allele components
Component
Use(s)
Regulatory region(s)
htt
Encoded product / tool
htt
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressor of
      Phenotype Manifest In
      Suppressor of
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference

      Expression of Hsap\MAPTScer\UAS.T:Avic\GFP under the control of Scer\GAL4shot-OK307 in the htt98E2 homozygous mutant background induces prominent collapse of the thorax due to severe loss of the muscles below, these phenotypes are not observed in either the Hsap\MAPTScer\UAS.T:Avic\GFP expressing flies or the htt98E2 mutants alone and can be rescued by combination with a single copy of htt+m22.7.

      The age-dependent loss of climbing ability as well as the reduced lifespan characteristic for htt98E2 homozygous adults is further worsened by expression of Hsap\MAPTScer\UAS.T:Avic\GFP under the control of Scer\GAL4shot-OK307 in the mutant background. This worsened phenotype can in turn be fully rescued by combination with a single copy of htt+m22.7.

      Expression of Hsap\MAPTScer\UAS.T:Avic\GFP under the Scer\GAL4shot-OK307 driver in Atg1Δ3D/+ heterozygous background leads to moderate thoracic collapse and loss of skeletal muscles, this phenotype is strongly enhanced by combination with a single copy of htt98E2.

      Hsap\MAPTScer\UAS.T:Avic\GFP expression in adults heterozygous for any of the following alleles: Atg13Δ81, Atg7d77 or ref(2)Pc03993 has no phenotype, unless the animals are also heterozygous for htt98E2, in which case a severe loss of thoracic somatic muscles is observed.

      (Rui et al., 2015)
      Complementation and Rescue Data
      Rescues

      htt+m22.7 rescues htt98E2

      (Rui et al., 2015)

      htt+m22.7 rescues htt98E2

      (Zhang et al., 2009)
      Comments

      The age-dependent loss of climbing ability as well as the reduced lifespan characteristic for htt98E2 homozygous adults is fully restored by combination with a single copy of htt+m22.7.

      (Rui et al., 2015)

      htt+m22.7 rescues the shortlived phenotype and the age-dependent mobility defects seen in htt98E2 animals (Df(3R)98E2 homozygotes in which CG9990 function has been rescued by P{CG9990+t24.7}).

      (Zhang et al., 2009)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      dhttRescue
      (Rui et al., 2015)
      htt+m22.7
      Name Synonyms
      Secondary FlyBase IDs
        References (3)