Full length htt is expressed under the control of its endogenous regulatory region. The 22.7kb minigene consists of a 14.9kb genomic fragment containing 5' regulatory sequences together with the first ten introns and exons, followed by a 6.6kb cDNA fragment that contains from exon 10 to exon 29, followed by a 1.2kb genomic fragment containing the exon 29 and the remaining polyA sites and untranscribed region at the 3' end of the gene.
Expression of Hsap\MAPTScer\UAS.T:Avic\GFP under the control of Scer\GAL4shot-OK307 in the htt98E2 homozygous mutant background induces prominent collapse of the thorax due to severe loss of the muscles below, these phenotypes are not observed in either the Hsap\MAPTScer\UAS.T:Avic\GFP expressing flies or the htt98E2 mutants alone and can be rescued by combination with a single copy of htt+m22.7.
The age-dependent loss of climbing ability as well as the reduced lifespan characteristic for htt98E2 homozygous adults is further worsened by expression of Hsap\MAPTScer\UAS.T:Avic\GFP under the control of Scer\GAL4shot-OK307 in the mutant background. This worsened phenotype can in turn be fully rescued by combination with a single copy of htt+m22.7.
Expression of Hsap\MAPTScer\UAS.T:Avic\GFP under the Scer\GAL4shot-OK307 driver in Atg1Δ3D/+ heterozygous background leads to moderate thoracic collapse and loss of skeletal muscles, this phenotype is strongly enhanced by combination with a single copy of htt98E2.
Hsap\MAPTScer\UAS.T:Avic\GFP expression in adults heterozygous for any of the following alleles: Atg13Δ81, Atg7d77 or ref(2)Pc03993 has no phenotype, unless the animals are also heterozygous for htt98E2, in which case a severe loss of thoracic somatic muscles is observed.