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General Information
Symbol
Dmel\htt+m22.7
Species
D. melanogaster
Name
FlyBase ID
FBal0241669
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

Full length htt is expressed under the control of its endogenous regulatory region. The 22.7kb minigene consists of a 14.9kb genomic fragment containing 5' regulatory sequences together with the first ten introns and exons, followed by a 6.6kb cDNA fragment that contains from exon 10 to exon 29, followed by a 1.2kb genomic fragment containing the exon 29 and the remaining polyA sites and untranscribed region at the 3' end of the gene.

Allele components
Product class / Tool use(s)
Regulatory region(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Phenotype Manifest In
Suppressor of
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference

Expression of Hsap\MAPTScer\UAS.T:Avic\GFP under the control of Scer\GAL4shot-OK307 in the htt98E2 homozygous mutant background induces prominent collapse of the thorax due to severe loss of the muscles below, these phenotypes are not observed in either the Hsap\MAPTScer\UAS.T:Avic\GFP expressing flies or the htt98E2 mutants alone and can be rescued by combination with a single copy of htt+m22.7.

The age-dependent loss of climbing ability as well as the reduced lifespan characteristic for htt98E2 homozygous adults is further worsened by expression of Hsap\MAPTScer\UAS.T:Avic\GFP under the control of Scer\GAL4shot-OK307 in the mutant background. This worsened phenotype can in turn be fully rescued by combination with a single copy of htt+m22.7.

Expression of Hsap\MAPTScer\UAS.T:Avic\GFP under the Scer\GAL4shot-OK307 driver in Atg1Δ3D/+ heterozygous background leads to moderate thoracic collapse and loss of skeletal muscles, this phenotype is strongly enhanced by combination with a single copy of htt98E2.

Hsap\MAPTScer\UAS.T:Avic\GFP expression in adults heterozygous for any of the following alleles: Atg13Δ81, Atg7d77 or ref(2)Pc03993 has no phenotype, unless the animals are also heterozygous for htt98E2, in which case a severe loss of thoracic somatic muscles is observed.

Complementation and Rescue Data
Comments

The age-dependent loss of climbing ability as well as the reduced lifespan characteristic for htt98E2 homozygous adults is fully restored by combination with a single copy of htt+m22.7.

htt+m22.7 rescues the shortlived phenotype and the age-dependent mobility defects seen in htt98E2 animals (Df(3R)98E2 homozygotes in which CG9990 function has been rescued by P{CG9990+t24.7}).

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
dhttRescue
htt+m22.7
Name Synonyms
Secondary FlyBase IDs
    References (3)