FlyBase Allele Report: Dmel\Mad[RNAi.UAS.226-807]

General Information

Symbol

Dmel\Mad^{RNAi.UAS.226-807}

Species

D. melanogaster

Name

FlyBase ID

FBal0242005

Feature type

allele

Associated gene

Dmel\Mad

Associated Insertion(s)

Carried in Construct

P{UAS-Mad.RNAi.226-807}

Also Known As

UAS-Mad RNAi

Key Links

Transgenic product class

RNAi_reagent

(Eivers et al., 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Eivers et al., 2009)

Mutagen

in vitro construct

(Eivers et al., 2009)

Progenitor genotype

Carried in construct

P{UAS-Mad.RNAi.226-807}

Cytology

Description

UAS regulatory sequences drive expression of an inverted repeat corresponding to nucleotides 226-807 of the coding region of Mad.

(Eivers et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Mad

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die before end of pupal stage, with Scer\GAL4^da.G32

(Eivers et al., 2009)

some die during pupal stage, with Scer\GAL4^da.G32

(Eivers et al., 2009)

visible, with Scer\GAL4^A9

(Eivers et al., 2009)

visible, with Scer\GAL4^Bx-MS1096-KE

(Eivers et al., 2009)

visible | somatic clone, with Scer\GAL4^Act5C.PI

(Eivers et al., 2009)

Phenotype Manifest In

anterior wing margin | somatic clone, with Scer\GAL4^Act5C.PI

(Eivers et al., 2009)

wing | temperature conditional, with Scer\GAL4^Bx-MS1096-KE

(Eivers et al., 2009)

wing margin bristle, with Scer\GAL4^A9

(Eivers et al., 2009)

wing vein, with Scer\GAL4^Bx-MS1096-KE

(Eivers et al., 2009)

Detailed Description

Statement

Reference

Clones of cells expressing Scer\GAL4^Act5C.PI-driven Mad^{dsRNA.Scer\UAS.cEa} can cause the loss of the anterior wing margin within clones.

When driven in the wing pouch with Scer\GAL4^A9, mechanosensory bristles are partially lost in the wing margin.

Driving Mad^{dsRNA.Scer\UAS.cEa} in the wing with Scer\GAL4^Bx-MS1096-KE reduced its size and eliminated veins at room temperature. Doubling the dose of Mad^{dsRNA.Scer\UAS.cEa} or driving Scer\GAL4^Bx-MS1096-KE at higher temperatures, results in flies lacking wings.

Pupae expressing Scer\GAL4^da.G32-driven Mad^{dsRNA.Scer\UAS.cEa} fail to eclose.

(Eivers et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Mad^{RNAi.UAS.226-807}, Scer\GAL4^Bx-MS1096-KE has visible phenotype, suppressible by Hsap\SMAD1^UAS.cMa, Scer\GAL4^Bx-MS1096-KE

(Eivers et al., 2009)

Mad^{RNAi.UAS.226-807}, Scer\GAL4^da.G32 has lethal | pupal stage phenotype, suppressible by Hsap\SMAD1^UAS.cMa, Scer\GAL4^da.G32

(Eivers et al., 2009)

Phenotype Manifest In

Suppressed by

Statement

Reference

Mad^{RNAi.UAS.226-807}, Scer\GAL4^Bx-MS1096-KE has wing phenotype, suppressible by Hsap\SMAD1^UAS.cMa, Scer\GAL4^Bx-MS1096-KE

(Eivers et al., 2009)

Mad^{RNAi.UAS.226-807}, Scer\GAL4^Bx-MS1096-KE has wing vein phenotype, suppressible by Hsap\SMAD1^UAS.cMa, Scer\GAL4^Bx-MS1096-KE

(Eivers et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Co-expression of Hsap\MADH1^Scer\UAS.cMa can rescue the wing size and vein development defects caused by Scer\GAL4^Bx-MS1096-KE-driven expression of Mad^{dsRNA.Scer\UAS.cEa}.

Hsap\MADH1^Scer\UAS.cMa can rescue the lethality of Scer\GAL4^da.G32-driven Mad^{dsRNA.Scer\UAS.cEa} expression.

(Eivers et al., 2009)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Expression of Mad^{dsRNA.Scer\UAS.cEa} produces dsRNA which results in dsRNA interference (RNAi) of the Mad gene.

(Eivers et al., 2009)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Mad^{RNAi.UAS.226-807}

Mad^RNAi.UAS.cEa

Mad^{dsRNA.Scer\UAS.cEa}

Mad^{dsRNA.UAS.cEa}

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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