FlyBase Allele Report: Dmel\Mad[MGM.UAS]

General Information

Symbol

Dmel\Mad^MGM.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0242010

Feature type

allele

Associated gene

Dmel\Mad

Associated Insertion(s)

Carried in Construct

P{UAS-Mad.MGM}

Key Links

Transgenic product class

missense_variant

(Eivers et al., 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Eivers et al., 2009)

Mutagen

in vitro construct

(Eivers et al., 2009)

Progenitor genotype

Carried in construct

P{UAS-Mad.MGM}

Cytology

Description

Amino acid replacement: S208A.

(Eivers et al., 2009)

UAS regulatory sequences drive expression of a mutant form of Mad, where serine 208 and 204 are mutated into alanines.

(Eivers et al., 2009)

Amino acid replacement: S204A.

(Eivers et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Mad

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible, with Scer\GAL4^A9

(Eivers et al., 2009)

visible, with Scer\GAL4^ap-md544

(Eivers et al., 2009)

visible, with Scer\GAL4^ptc-559.1

(Eivers et al., 2009)

visible, with Scer\GAL4^sca-537.4

(Quijano et al., 2011)

visible, with Scer\GAL4^sd-SG29.1

(Eivers et al., 2009)

visible | embryonic stage, with Scer\GAL4^{VP16.mat.αTub67C}

(Eivers et al., 2009)

visible | somatic clone, with Scer\GAL4^Act5C.PI

(Eivers et al., 2009)

Phenotype Manifest In

crossvein | ectopic, with Scer\GAL4^ptc-559.1

(Eivers et al., 2009)

dorsal row of triple row of wing sensilla | ectopic, with Scer\GAL4^sd-SG29.1

(Eivers et al., 2009)

dorsal row of triple row of wing sensilla | increased number, with Scer\GAL4^A9

(Eivers et al., 2009)

dorsal row of triple row of wing sensilla | increased number, with Scer\GAL4^sd-SG29.1

(Eivers et al., 2009)

medial row of triple row of wing sensilla | increased number, with Scer\GAL4^A9

(Eivers et al., 2009)

medial row of triple row of wing sensilla | increased number, with Scer\GAL4^sd-SG29.1

(Eivers et al., 2009)

ventral denticle belt, with Scer\GAL4^{VP16.mat.αTub67C}

(Eivers et al., 2009)

wing margin | somatic clone, with Scer\GAL4^Act5C.PI

(Eivers et al., 2009)

wing margin bristle | ectopic, with Scer\GAL4^sca-537.4

(Quijano et al., 2011)

wing pouch, with Scer\GAL4^sd-SG29.1

(Eivers et al., 2009)

wing sensillum | ectopic, with Scer\GAL4^sca-537.4

(Quijano et al., 2011)

wing vein | ectopic, with Scer\GAL4^ptc-559.1

(Eivers et al., 2009)

wing vein | ectopic, with Scer\GAL4^sca-537.4

(Quijano et al., 2011)

Detailed Description

Statement

Reference

Expression of Mad^MGM.Scer\UAS under the control of Scer\GAL4^sca-537.4 results in a variety of wing phenotypes including wild-type wings, ectopic veins, ectopic sensilla on the anterior crossvein, L1 and L3 veins and dorsal chemosensory bristle duplications.

(Quijano et al., 2011)

Scer\GAL4^ptc-559.1-driven expression of Mad^MGM.Scer\UAS induces a crossvein-like phenotype. When driven in the dorsal wing compartment with Scer\GAL4^ap-md544, Mad^MGM.Scer\UAS induces large amounts of ectopic vein tissue, accompanied by blistering.

Overexpression of Mad^MGM.Scer\UAS driven by Scer\GAL4^sd-SG29.1 or Scer\GAL4^A9 results in additional sensory bristles, both stout mechanosensory and chemosensory bristles are formed in the wing margin.

When driven by Scer\GAL4^sd-SG29.1, Mad^MGM.Scer\UAS can induce chemosensory bristles on the wing blade itself in the absence of ectopic vein tissue.

Clones of cells overexpressing Mad^MGM.Scer\UAS driven by Scer\GAL4^Act5C.PI can cause duplications of the wing margin.

Scer\GAL4^sd-SG29.1-driven Mad^MGM.Scer\UAS increases the size of the wing pouch.

Overexpression of Mad^MGM.Scer\UAS driven by Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} causes patches of naked cuticle at the expense of denticle rows.

(Eivers et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Mad^MGM.Scer\UAS

Mad^MGM.UAS

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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