FlyBase Allele Report: Dmel\ppk[JF03250]

General Information

Symbol

Dmel\ppk^JF03250

Species

D. melanogaster

Name

FlyBase ID

FBal0242426

Feature type

allele

Associated gene

Dmel\ppk

Associated Insertion(s)

Carried in Construct

P{TRiP.JF03250}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Perkins et al., 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Perkins et al., 2009)

Mutagen

in vitro construct

(Perkins et al., 2009)

Progenitor genotype

Carried in construct

P{TRiP.JF03250}

Cytology

Description

UAS regulatory sequences drive expression of an inverted repeat.

(Perkins et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-JF03250

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Machado-Joseph disease

modeled by Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}

(McGurk and Bonini, 2012)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | third instar larval stage, with Scer\GAL4^109(2)80

(Jakubowski et al., 2012)

abnormal locomotor behavior | third instar larval stage, with Scer\GAL4^ppk.PG

(Jang et al., 2019)

abnormal pain response | third instar larval stage, with Scer\GAL4^ppk.PG

(Jang et al., 2019)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of ppk^JF03250 in third instar larvae under the control of Scer\GAL4^ppk.PG leads to impaired nociceptive responses to harsh mechanical forces (45mN) and fewer turns during movement when compared to controls.

(Jang et al., 2019)

Third instar larvae expressing ppk^JF03250 under the control of Scer\GAL4^109(2)80 crawl faster and are less likely to switch directions compared to wild-type.

(Jakubowski et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Scer\GAL4^ppk.PG, ppk^JF03250 has abnormal pain response | third instar larval stage phenotype, non-enhanceable by ppk30^KK104053/Scer\GAL4^ppk.PG

(Jang et al., 2019)

Scer\GAL4^ppk.PG, ppk^JF03250 has abnormal locomotor behavior | third instar larval stage phenotype, non-enhanceable by ppk30^KK104053/Scer\GAL4^ppk.PG