FlyBase Allele Report: Dmel\CalpA[JF03391]

General Information

Symbol

Dmel\CalpA^JF03391

Species

D. melanogaster

Name

FlyBase ID

FBal0242563

Feature type

allele

Associated gene

Dmel\CalpA

Associated Insertion(s)

Carried in Construct

P{TRiP.JF03391}

Also Known As

UAS-CalpA RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Perkins et al., 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Perkins et al., 2009)

Mutagen

in vitro construct

(Perkins et al., 2009)

Progenitor genotype

Carried in construct

P{TRiP.JF03391}

Cytology

Description

UAS regulatory sequences drive expression of an inverted repeat.

(Perkins et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-JF03391

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of intellectual disability

CEA

(Haddadi and Ataei, 2022)

model of obesity

CEA

(Agrawal et al., 2021)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates amyotrophic lateral sclerosis type 10

modeled by TBPH^{UAS.Tag:FLAG.Tag:HA}

(Park et al., 2020)

ameliorates Machado-Joseph disease

modeled by Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}

(Park et al., 2020)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

A1-7 lateral transverse muscle cell, with Scer\GAL4^Mef2.PR

(Buffolo et al., 2015)

dendrite | pupal stage, with Scer\GAL4^ppk.PG

(Metwally et al., 2021)

larval multidendritic class IV neuron | pupal stage, with Scer\GAL4^ppk.PG

(Metwally et al., 2021)

Detailed Description

Statement

Reference

The expression of CalpA^JF03391 under the control of Scer\GAL4^ppk.PG results in dendrite pruning defects in class IV da neurons at 14h APF.

(Metwally et al., 2021)

Expression of CalpA^GS9103 under the control of Scer\GAL4^Mef2.PR results in a lethal embryonic phenotype. This is partially suppressed by co-expression of CalpA^JF03391.

Expression of CalpA^JF03391 under the control of Scer\GAL4^Mef2.PR (with UAS-Dicer2 to increase RNAi efficiency) results in locomotion defects in third instar larvae; there is a significant reduction in speed and a significant increase in the time taken for larvae to right themselves. Expression under the control of Scer\GAL4^C23 also results in locomotion defects in third instar larvae.

The length and width of the lateral transverse muscles is significantly reduced in embryos expressing CalpA^JF03391 under the control of Scer\GAL4^Mef2.PR.

(Buffolo et al., 2015)

Adults expressing CalpA^JF03391 under the control of Scer\GAL4^Cg.PA show a reduced survival rate compared to control flies when challenged with the fungus Beauveria bassiana.

The survival rate of adults expressing CalpA^JF03391 under the control of Scer\GAL4^Cg.PA when challenged with E. coli is not significantly different from that of controls.

(Fontenele et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

CalpA^JF03391, Scer\GAL4^ppk.PG has abnormal neuroanatomy | pupal stage phenotype, suppressible by ttm50^UAS.Tag:HA, Scer\GAL4^ppk.PG

(Metwally et al., 2021)

CalpA^JF03391, Scer\GAL4^ppk.PG has increased size | pupal stage phenotype, suppressible by ttm50^UAS.Tag:HA, Scer\GAL4^ppk.PG