FlyBase Allele Report: Dmel\RASSF8[6]

General Information

Symbol

Dmel\RASSF8⁶

Species

D. melanogaster

Name

FlyBase ID

FBal0242639

Feature type

allele

Associated gene

Dmel\RASSF8

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Langton et al., 2009)

Progenitor genotype

P{EP}GE15974

(Langton et al., 2009)

Caused by aberration

Df(3R)RASSF8⁶

Cytology

Description

Imprecise excision of the progenitor insertion, resulting in a 1.4kb deletion that removes the first exon and part of the second exon of RASSF8. 81bp of the 5' untranslated region of the neighbouring tankyrase gene is also deleted, but the level of the tankyrase transcript is not obviously affected.

(Langton et al., 2009)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased cell death

(Langton et al., 2009)

decreased cell death | somatic clone

(Langton et al., 2009)

increased cell number

(Langton et al., 2009)

visible

(Langton et al., 2009)

Phenotype Manifest In

eye

(Langton et al., 2009)

interommatidial bristle

(Langton et al., 2009)

interommatidial cell

(Langton et al., 2009)

interommatidial cell | pupal stage | increased number

(Zaessinger et al., 2015)

ommatidium

(Langton et al., 2009)

wing

(Langton et al., 2009)

Detailed Description

Statement

Reference

RASSF8⁶ mutants have slightly rounder wings and show a significant increase in the number of inter-ommatidial cells in the pupal eye disc.

(Zaessinger et al., 2015)

Homozygotes have a broad wing phenotype, with the wings being shorter along the proximodistal axis and broader along the anteroposterior axis compared to wild-type controls.

The average wing area of homozygotes is 5.8% larger than that of controls, while the wing hair density is comparable to that of controls, indicating that the wings contain more cells rather than larger cells. The adult body weight of homozygous flies is 10.5% higher than that of control flies.

Homozygotes have enlarged, rough eyes. The hexagonal ommatidial lattice is disrupted and there is clustering of interommatidial bristles. Extra interommatidial cells are seen in the retinas of mutant pupae, with a double layer of these cells often being seen. The mutant pupal retinas show a significant decrease in apoptosis compared to controls. Homozygous clones in the pupal retina also show a significant decrease in apoptosis compared to control clones.

(Langton et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

RASSF8⁶ has visible phenotype, enhanceable by shg[+]/shg^R69

(Langton et al., 2009)

Suppressor of

Statement

Reference

RASSF8[+]/RASSF8⁶ is a suppressor of visible | adult stage phenotype of Magi^UAS.GFP, Scer\GAL4^GMR.PU

(Zaessinger et al., 2015)

Phenotype Manifest In

Enhanced by

Statement

Reference

RASSF8⁶ has eye phenotype, enhanceable by shg[+]/shg^R69

(Langton et al., 2009)

Suppressor of

Statement

Reference

RASSF8[+]/RASSF8⁶ is a suppressor of eye | adult stage phenotype of Magi^UAS.GFP, Scer\GAL4^GMR.PU

(Zaessinger et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

The rough eye phenotype in adult flies induced by expression of Magi^{Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^GMR.PU is significantly suppressed by combination with a single copy of RASSF8⁶.

(Zaessinger et al., 2015)

The enlarged eye phenotype seen in RASSF8⁶ homozygotes is enhanced by shg^R69/+.

(Langton et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

RASSF8⁶ is rescued by RASSF8^Ubi-p63E.PZ

(Zaessinger et al., 2015)

RASSF8⁶ is rescued by RASSF8^UASp.cLa/Scer\GAL4^arm.PS

(Langton et al., 2009)

Partially rescued by

RASSF8⁶ is partially rescued by RASSF8^YA.Ubi-p63E

(Zaessinger et al., 2015)

Comments

The increased number of inter-ommatidial cells characteristic for RASSF8⁶ flies is fully rescues by expression of RASSF8^Ubi-p63E.PZ in the mutant background.

The increased number of inter-ommatidial cells characteristic for RASSF8⁶ flies is fully rescues by expression of RASSF8^YA.Ubi-p63E in the mutant background.

(Zaessinger et al., 2015)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

RASSF8⁶

(Chan et al., 2021, Zaessinger et al., 2015)

dRASSF8⁶

(Langton et al., 2009)

rassf8⁶

(Höhne et al., 2025)

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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