2kb genomic fragment that encompasses His3.3A, mutated to carry an S31A amino acid substitution. Two copies of the Tag:FLAG epitope have been inserted at the N-terminal end of the coding sequence.
His3.3A2X1, His3.3AS31A.Tag:FLAG, His3.3B0 has viable phenotype
His3.3A2X1, His3.3AS31A.Tag:FLAG, His3.3B0 has male fertile phenotype
His3.3A2X1, His3.3B0 double mutant males also carrying a His3.3AS31A.T:Zzzz\FLAG transgene are viable and fertile.