FlyBase Allele Report: Dmel\His3.3B[0]

General Information

Symbol

Dmel\His3.3B⁰

Species

D. melanogaster

Name

FlyBase ID

FBal0242712

Feature type

allele

Associated gene

Dmel\His3.3B

Associated Insertion(s)

Carried in Construct

Also Known As

H3.3B⁰

Key Links

Allele class

amorphic allele - molecular evidence

Mutagen

FLPase

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Sakai et al., 2009)

Mutagen

FLPase

(Sakai et al., 2009)

Progenitor genotype

PBac{RB}e02425

(Sakai et al., 2009)

P{XP}His3.3B^d04051

(Sakai et al., 2009)

Cytology

Description

A deletion removing His3.3B, generated by Scer\FLP1-mediated recombination between P{XP}His3.3B^d04051 and PBac{RB}e02425.

(Sakai et al., 2009)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

His3.3A^2X1, His3.3B⁰ has partially lethal - majority die phenotype

(Sakai et al., 2009)

His3.3A^2X1, His3.3A^K9R.Tag:FLAG, His3.3B⁰ has male fertile phenotype

(Sakai et al., 2009)

His3.3A^2X1, His3.3A^{S31A.Tag:FLAG}, His3.3B⁰ has viable phenotype

(Sakai et al., 2009)

His3.3A^2X1, His3.3A^{S31A.Tag:FLAG}, His3.3B⁰ has male fertile phenotype

(Sakai et al., 2009)

His3.3A^2X1, His3.3B⁰ has sterile phenotype

(Sakai et al., 2009)

His3.3A^2X1, His3.3A^Tag:FLAG.cSa, His3.3B⁰ has viable phenotype

(Sakai et al., 2009)

His3.3A^2X1, His3.3A^Tag:FLAG.cSa, His3.3B⁰ has male fertile phenotype

(Sakai et al., 2009)

His3.3A^2X1, His3.3A^H3.Tag:FLAG, His3.3B⁰ has viable phenotype

(Sakai et al., 2009)

His3.3A^2X1, His3.3A^H3.Tag:FLAG, His3.3B⁰ has male sterile phenotype

(Sakai et al., 2009)

His3.3A^2X1, His3.3A^K4R.Tag:FLAG, His3.3B⁰ has partially lethal - majority die phenotype

(Sakai et al., 2009)

His3.3A^2X1, His3.3A^K4R.Tag:FLAG, His3.3B⁰ has male fertile phenotype

(Sakai et al., 2009)

His3.3A^2X1, His3.3A^K9R.Tag:FLAG, His3.3B⁰ has partially lethal - majority die phenotype

(Sakai et al., 2009)

Phenotype Manifest In

Other

Statement

Reference

His3.3A^2X1, His3.3B⁰ has spermatid phenotype

(Sakai et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

His3.3A^2X1, His3.3B⁰ double mutants have reduced viability and are invariably sterile in both males and females.

Round developing spermatids are present in testes of His3.3A^2X1, His3.3B⁰ double mutant males but vary in size. Late elongating nuclei are never observed in the double mutants. Clear defects in meiosis are apparent by metaphase I, with diffuse chromosomes that are only partially condensed. Metaphase I with incompletely condensed chromosomes is followed by an aberrant anaphase in the double mutants. Further meiotic aberrations occur in anaphase II in His3.3A^2X1, His3.3B⁰ double mutant testes, such as chromosome bridges between sister chromatids. Compared to wild-types, His3.3A^2X1, His3.3B⁰ double mutant testes contain postmeiotic nuclei that are abnormal in size. The postmeiotic nuclear body is often fragmented into two-fifteen smaller bodies in the double mutants.

His3.3A^2X1, His3.3B⁰ double mutant males also carrying a His3.3A^{T:Zzzz\FLAG.cSa} transgene are viable and fertile. The His3.3A^{T:Zzzz\FLAG.cSa} rescue construct restores all aspects of normal germ lines (meiotic segregation, the single nuclear body in postmeiotic nuclei, production of motile sperm and male fertility) to double mutant males.

His3.3A^2X1, His3.3B⁰ double mutant males also carrying a His3.3A^{H3.T:Zzzz\FLAG} transgene are viable, but sterile. Meiotic defects and fragmentation of the postmeiotic nuclear body persists in His3.3A^2X1, His3.3B⁰ double mutant males carrying His3.3A^{H3.T:Zzzz\FLAG}.

His3.3A^2X1, His3.3B⁰ double mutant males also carrying a His3.3A^{K4R.T:Zzzz\FLAG} transgene are fertile, but they show reduced viability. The His3.3A^2X1, His3.3B⁰ double mutant germ line cells also carrying His3.3A^{K4R.T:Zzzz\FLAG} show no defects.

His3.3A^2X1, His3.3B⁰ double mutant males also carrying a His3.3A^{K9R.T:Zzzz\FLAG} transgene are fertile, but they show reduced viability.

His3.3A^2X1, His3.3B⁰ double mutant males also carrying a His3.3A^{S31A.T:Zzzz\FLAG} transgene are viable and fertile.

(Sakai et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

68240

y¹ w^* His3.3B⁰; His3.3A^2X1/CyO, S²

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

H3.3B⁰

(McPherson et al., 2023, Salzler et al., 2023, Leatham-Jensen et al., 2019)

His3.3B⁰

(Schneiderman et al., 2012, Sakai et al., 2009)

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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