His3.3A2X1, His3.3ATag:FLAG.cSa, His3.3B0 has viable phenotype
His3.3A2X1, His3.3ATag:FLAG.cSa, His3.3B0 has male fertile phenotype
His3.3A2X1, His3.3B0 double mutant males also carrying a His3.3AT:Zzzz\FLAG.cSa transgene are viable and fertile. The His3.3AT:Zzzz\FLAG.cSa rescue construct restores all aspects of normal germ lines (meiotic segregation, the single nuclear body in postmeiotic nuclei, production of motile sperm and male fertility) to double mutant males.