FlyBase Allele Report: Dmel\Cln3[UAS.Venus]

General Information

Symbol

Dmel\Cln3^UAS.Venus

Species

D. melanogaster

Name

FlyBase ID

FBal0243515

Feature type

allele

Associated gene

Dmel\Cln3

Associated Insertion(s)

Carried in Construct

P{UAS-Cln3.Venus}

Key Links

Transgenic product class

wild_type

(Tuxworth et al., 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Tuxworth et al., 2009)

Mutagen

in vitro construct

(Tuxworth et al., 2009)

Progenitor genotype

Carried in construct

P{UAS-Cln3.Venus}

Cytology

Description

UAS regulatory sequences drive expression of wild-type Cln3 coding sequences tagged with Venus.

(Tuxworth et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Cln3

Tagged with

Venus

yellow fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PF

(Tuxworth et al., 2009)

ommatidium, with Scer\GAL4^GMR.PF

(Tuxworth et al., 2009)

Detailed Description

Statement

Reference

Expression of cln3^{Scer\UAS.T:Avic\GFP-YFP.Venus} in the eye, under the control of Scer\GAL4^GMR.PF results in misshapen and fused ommatidia. Scanning electron microscopy shows a loss of regular crystalline array of ommatidia, fusions of ommatidia and alterations in bristle patterns. Sections through the eye show extensive degeneration.

(Tuxworth et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

Cln3^UAS.Venus, Scer\GAL4^GMR.PF has ommatidium phenotype, enhanceable by Df(2R)ED3791, Scer\GAL4^GMR.PF

(Tuxworth et al., 2009)

Cln3^UAS.Venus, Scer\GAL4^GMR.PF has eye phenotype, enhanceable by Df(2R)ED3791, Scer\GAL4^GMR.PF

(Tuxworth et al., 2009)

Suppressor of

Statement

Reference

Cln3^UAS.Venus, Scer\GAL4^GMR.PU is a suppressor | partially of ommatidium phenotype of Ppt1^UAS.cKa, Scer\GAL4^GMR.PU

(Jones et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

A Df(2R)ED3791 background enhances eye degeneration found in Scer\GAL4^GMR.PF>cln3^{Scer\UAS.T:Avic\GFP-YFP.Venus} flies.

(Tuxworth et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Cln3^UAS.Venus rescues Cln3^ΔMB1

(Tuxworth et al., 2011)

Comments

Ubiquitous expression of cln3^{Scer\UAS.T:Avic\GFP-YFP.Venus} under the control of Scer\GAL4^arm.PS rescues the hypersensitivity of cln3^ΔMB1 mutants to oxidative stress. The shortened lifespan of cln3^ΔMB1 homozygotes is also rescued by the expression of cln3^{Scer\UAS.T:Avic\GFP-YFP.Venus} via Scer\GAL4^arm.PS.

(Tuxworth et al., 2011)

A Df(3R)3-4 background suppresses eye degeneration found in Scer\GAL4^GMR.PF>cln3^{Scer\UAS.T:Avic\GFP-YFP.Venus} flies.

(Tuxworth et al., 2009)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Cln3^{Scer\UAS.T:Avic\GFP-YFP.Venus}

Cln3^UAS.Venus

cln3^{Scer\UAS.T:Avic\GFP-YFP.Venus}

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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