FlyBase Allele Report: Hsap\CLN3[UAS.cTa]

General Information

Symbol

Hsap\CLN3^UAS.cTa

Species

H. sapiens

Name

FlyBase ID

FBal0243518

Feature type

allele

Associated gene

Hsap\CLN3

Associated Insertion(s)

Carried in Construct

P{UAS-HsCln3.T}

Key Links

Transgenic product class

wild_type

(Tuxworth et al., 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Tuxworth et al., 2009)

Mutagen

in vitro construct

(Tuxworth et al., 2009)

Progenitor genotype

Carried in construct

P{UAS-HsCln3.T}

Cytology

Description

UAS regulatory sequences drive expression of Hsap\CLN3 coding sequences.

(Tuxworth et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Hsap\CLN3

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

partially lethal - majority die, with Scer\GAL4^da.G32

(Tuxworth et al., 2009)

Phenotype Manifest In

eye, with Scer\GAL4^da.G32

(Tuxworth et al., 2009)

eye, with Scer\GAL4^GMR.PF

(Tuxworth et al., 2009)

macrochaeta, with Scer\GAL4^da.G32

(Tuxworth et al., 2009)

ommatidium, with Scer\GAL4^GMR.PF

(Tuxworth et al., 2009)

scutellar bristle | increased number, with Scer\GAL4^en-e16E

(Tuxworth et al., 2009)

sternopleural bristle, with Scer\GAL4^dpp.blk1

(Tuxworth et al., 2009)

wing, with Scer\GAL4^da.G32

(Tuxworth et al., 2009)

wing, with Scer\GAL4^vg.M.Exel

(Tuxworth et al., 2009)

wing blade, with Scer\GAL4^vg.M.Exel

(Tuxworth et al., 2009)

wing margin, with Scer\GAL4^da.G32

(Tuxworth et al., 2009)

wing margin, with Scer\GAL4^vg.M.Exel

(Tuxworth et al., 2009)

wing vein, with Scer\GAL4^da.G32

(Tuxworth et al., 2009)

Detailed Description

Statement

Reference

Expression of Hsap\CLN3^Scer\UAS.cTa in the eye under the control of Scer\GAL4^GMR.PF results in disruption to ommatidial organization and widespread vacuolation, indicative of degeneration.

Ubiquitous overexpression of Hsap\CLN3^Scer\UAS.cTa by Scer\GAL4^da.G32 is semi-lethal. Escaping adults are noticeably small than controls and display roughened eyes. Overexpression also leads to duplication of macrochaetae on the thorax of the adult fly. Wings display thickened veins, vein deltas and notches in the wing margin.

Expression of Hsap\CLN3^Scer\UAS.cTa by Scer\GAL4^dpp.blk1 results in duplications in the sternopleural bristles on the side of the adult fly.

Expression of Hsap\CLN3^Scer\UAS.cTa in the wing margin by Scer\GAL4^vg.M.Exel causes large notches to appear in the wing blade.

Overexpression of Hsap\CLN3^Scer\UAS.cTa in the posterior of the imaginal wing disc using Scer\GAL4^en-e16E results in duplication of one or more bristles on the scutellum, with the mean number increasing compared to controls.

(Tuxworth et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

Hsap\CLN3^UAS.cTa, Scer\GAL4^en-e16E has scutellar bristle | increased number phenotype, enhanceable by Delta^RF/Dl[+], Scer\GAL4^en-e16E

(Tuxworth et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Overexpression of Hsap\CLN3^Scer\UAS.cTa in the posterior of the imaginal wing disc using Scer\GAL4^en-e16E in a heterozygous Dl^RF background results in increased duplication of one or more bristles on the scutellum, with the mean number increased compared to single mutants and controls.

(Tuxworth et al., 2009)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Hsap\CLN3^Scer\UAS.cTa

Hsap\CLN3^UAS.cTa

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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