FlyBase Allele Report: Dmel\mir-124[UAS.cXa]

General Information

Symbol

Dmel\mir-124^UAS.cXa

Species

D. melanogaster

Name

FlyBase ID

FBal0243579

Feature type

allele

Associated gene

Dmel\mir-124

Associated Insertion(s)

Carried in Construct

P{UAS-pre-mir-124a}

Key Links

Transgenic product class

wild_type

(Xu et al., 2008)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Xu et al., 2008)

Mutagen

in vitro construct

(Xu et al., 2008)

Progenitor genotype

Carried in construct

P{UAS-pre-mir-124a}

Cytology

Description

UASt regulatory sequences drive expression of a 92bp genomic DNA fragment containing the 77 nucleotide pre- mir-124 sequence.

(Xu et al., 2008)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

mir-124

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4²²¹

(Xu et al., 2008)

abnormal neuroanatomy, with Scer\GAL4⁴⁷⁷

(Xu et al., 2008)

Phenotype Manifest In

abdominal 1 dorsal multidendritic neuron ddaC, with Scer\GAL4⁴⁷⁷

(Xu et al., 2008)

abdominal 1 dorsal multidendritic neuron ddaE, with Scer\GAL4²²¹

(Xu et al., 2008)

abdominal 1 dorsal multidendritic neuron ddaF, with Scer\GAL4²²¹

(Xu et al., 2008)

dendrite, with Scer\GAL4²²¹

(Xu et al., 2008)

dendrite, with Scer\GAL4⁴⁷⁷

(Xu et al., 2008)

dendritic arborizing neuron, with Scer\GAL4²²¹

(Xu et al., 2008)

Detailed Description

Statement

Reference

Ectopic expression of mir-124^Scer\UAS.cXa in ddaE, ddaF and DA neurons in the dorsal cluster under the control of Scer\GAL4²²¹ results in significantly fewer dendritic ends than in wild-type neurons.

Ectopic expression of mir-124^Scer\UAS.cXa in ddaC neurons (under the control of Scer\GAL4⁴⁷⁷) decreases the number of dendritic ends and the size of the dendritic field compared with wild-type neurons.

(Xu et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4⁴⁷⁷, mir-124^UAS.cXa has abnormal neuroanatomy phenotype, suppressible by Dcr-1^Q1147X

(Xu et al., 2008)

Scer\GAL4⁴⁷⁷, mir-124^UAS.cXa has abnormal neuroanatomy phenotype, suppressible by Dcr-1^Q1948X

(Xu et al., 2008)

Scer\GAL4⁴⁷⁷, mir-124^UAS.cXa has abnormal neuroanatomy phenotype, suppressible | partially by Fmr1³

(Xu et al., 2008)

Scer\GAL4⁴⁷⁷, mir-124^UAS.cXa has abnormal neuroanatomy phenotype, suppressible | partially by Fmr1^4.L/Fmr1³

(Xu et al., 2008)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4⁴⁷⁷, mir-124^UAS.cXa has abdominal 1 dorsal multidendritic neuron ddaC phenotype, suppressible | partially by Fmr1^4.L/Fmr1³

(Xu et al., 2008)

Scer\GAL4⁴⁷⁷, mir-124^UAS.cXa has dendrite phenotype, suppressible | partially by Fmr1^4.L/Fmr1³

(Xu et al., 2008)

Scer\GAL4⁴⁷⁷, mir-124^UAS.cXa has abdominal 1 dorsal multidendritic neuron ddaC phenotype, suppressible | partially by Fmr1³

(Xu et al., 2008)

Scer\GAL4⁴⁷⁷, mir-124^UAS.cXa has dendrite phenotype, suppressible | partially by Fmr1³

(Xu et al., 2008)

Scer\GAL4⁴⁷⁷, mir-124^UAS.cXa has abdominal 1 dorsal multidendritic neuron ddaC phenotype, suppressible by Dcr-1^Q1948X

(Xu et al., 2008)

Scer\GAL4⁴⁷⁷, mir-124^UAS.cXa has dendrite phenotype, suppressible by Dcr-1^Q1948X

(Xu et al., 2008)

Scer\GAL4⁴⁷⁷, mir-124^UAS.cXa has abdominal 1 dorsal multidendritic neuron ddaC phenotype, suppressible by Dcr-1^Q1147X

(Xu et al., 2008)

Scer\GAL4⁴⁷⁷, mir-124^UAS.cXa has dendrite phenotype, suppressible by Dcr-1^Q1147X

(Xu et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

Ectopic expression of mir-124^Scer\UAS.cXa in ddaC neurons (under the control of Scer\GAL4⁴⁷⁷) in a homozygous Fmr1³ or Fmr1³/Fmr1^4.L background results in a significantly attenuated phenotype, where the number of dendritic ends is partially rescued (although not to the wild-type level). Interestingly, the dendritic fields of ddaC neurons are similar in size to those of wild-type neurons.

Ectopic expression of mir-124^Scer\UAS.cXa in homozygous Dcr-1^d102 or Dcr-1^Q1147X ddaC neuron clones (under the control of Scer\GAL4⁴⁷⁷) does not affect the number of dendritic ends, indicating Dcr-1 suppression of mir-124 function.

(Xu et al., 2008)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

mir-124^Scer\UAS.cXa

mir-124^UAS.cXa

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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