FB2026_02 , released June 18, 2026
Allele: Dmel\robo1KO.robo3
Open Close
General Information
Symbol
Dmel\robo1KO.robo3
Species
D. melanogaster
Name
FlyBase ID
FBal0243804
Feature type
allele
Associated gene
Dmel\robo1
Associated Insertion(s)
TI{TI}robo1KO.robo3
Carried in Construct
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
recombination
(Spitzweck et al., 2010)
I-SceI
(Spitzweck et al., 2010)
FLPase
(Spitzweck et al., 2010)
I-CreI
(Spitzweck et al., 2010)
ends-in gene targeting
(Spitzweck et al., 2010)
Progenitor genotype
Associated Insertion(s)
TI{TI}robo1KO.robo3
Cytology
Description

The exons (and intervening small introns) that encode the mature robo1 protein have been replaced with a single exon encoding full length robo3 tagged with Tag:HA. The robo1KO.robo3 allele represents the loss of the robo1 open reading frame at the endogenous robo1 locus, while the robo3robo1.HR.Tag:HA allele represents the mature robo3 protein tagged with Tag:HA that is expressed under the control of the robo1 regulatory sequences in place of robo1.

(Spitzweck et al., 2010)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Fas2-positive longitudinal fascicles of stage 16 roboKO.robo3 robo3robo.HR.T:Ivir\HA1 double homozygous embryos show midline crossing defects that are qualitatively and quantitatively similar to those seen in robo mutant embryos (Fas2-positive longitudinal fascicles extend across or along the midline in 99.2% of segments). In stage 13 roboKO.robo3 robo3robo.HR.T:Ivir\HA1 double homozygous embryos, the pCC axons project aberrantly across the midline, as occurs in robo mutant embryos.

      (Spitzweck et al., 2010)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      robo1KO.robo3
      robo1robo3
      (Spitzweck et al., 2010)
      roboKO.robo3
      Name Synonyms
      Secondary FlyBase IDs
        References (1)