FlyBase Allele Report: Dmel\Vps35[1]

General Information

Symbol

Dmel\Vps35¹

Species

D. melanogaster

Name

FlyBase ID

FBal0244072

Feature type

allele

Associated gene

Dmel\Vps35

Associated Insertion(s)

Carried in Construct

Also Known As

Dvps35¹

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Belenkaya et al., 2008)

Progenitor genotype

P{EPgy2}Vps35^EY14200

(Belenkaya et al., 2008)

Cytology

Description

Imprecise excision of P{EPgy2}Vps35^EY14200 resulting in a 1220 bp deletion removing the start codon and the first three exons of Vps35.

(Belenkaya et al., 2008)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

2R:22,186,070..22,187,289 [+]

Comment:

1220 bp deletion resulting from imprecise excision of P{EPgy2}Vps35^EY14200. Deletion location approximate. Reported as 1220 bp deletion removing the start codon and first three exons.

(Belenkaya et al., 2008)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Unlike wild-type type II neuroblast clones, which contain only one Dpn+ Ase- neuroblast, Vps35¹ mutant clones contain multiple ectopic Dpn+ Ase- Pros- neuroblast-like cells several cell diameters away from the primary neuroblast, which are of intermediate cell sizes between neural progenitors and primary neuroblasts.

(Li et al., 2018)

Vps35¹/Vps35^MH20 transheterozygotes are lethal at the prepupal stages. Their third instar larval neuromuscular junction exhibit a significant decrease in the size of synaptic boutons and reciprocal increase in the number of type Ib boutons (despite of insignificant changes in the number of active zones per bouton), a significant decrease in the synaptic pool, associated with the reciprocal significant decrease in number and increase in size of synaptic vesicles at active zones, a significant decrease in endocytosis, associated with a significant increase in the number of endocytic intermediates per active zone, and subtle ultrastructural defects at the subsynaptic reticulum, as compared to controls; these neuromuscular junctions also exhibit neurotransmission defects, namely a significant increase in miniature excitatory junction potential amplitude, a significant decrease in quantal content under low stimulation conditions, despite of insignificant changes in both excitatory junction potential amplitude and in paired-pulse ratio, and a significant decrease in excitatory junction potential amplitude under high stimulation conditions, as compared to controls.

Vps35¹ heterozygous adults exhibit a significant decrease in sleep bouts, as compared to controls.

(Inoshita et al., 2017)

Average dorsal trunk tube length is increased in stage 16 homozygous embryos compared to wild type.

(Dong et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Vps35¹ has abnormal circadian behavior | dominant | adult stage phenotype, suppressible by Lrrk[+]/Lrrk^e03680

(Inoshita et al., 2017)

Vps35^MH20/Vps35¹ has abnormal size | third instar larval stage phenotype, suppressible by Rab5^UAS.GFP/Scer\GAL4^da.G32

(Inoshita et al., 2017)

Vps35^MH20/Vps35¹ has abnormal size | third instar larval stage phenotype, suppressible by Rab11^{UAS.ORF.Tag:HA}/Scer\GAL4^da.G32

(Inoshita et al., 2017)

Vps35^MH20/Vps35¹ has abnormal size | third instar larval stage phenotype, suppressible by Lrrk^UAS.cIa/Scer\GAL4^da.G32

(Inoshita et al., 2017)

Vps35^MH20/Vps35¹ has abnormal size | third instar larval stage phenotype, suppressible by Lrrk[+]/Lrrk^e03680

(Inoshita et al., 2017)

Vps35¹ has abnormal circadian behavior | dominant | adult stage phenotype, suppressible by Rab5^UAS.GFP/Scer\GAL4^ple.PF

(Inoshita et al., 2017)

Vps35¹ has abnormal circadian behavior | dominant | adult stage phenotype, suppressible by Scer\GAL4^ple.PF/Rab11^{UAS.ORF.Tag:HA}

(Inoshita et al., 2017)

Vps35¹ has abnormal circadian behavior | dominant | adult stage phenotype, suppressible by Scer\GAL4^ple.PF/Lrrk^UAS.cIa

(Inoshita et al., 2017)

NOT suppressed by

Statement

Reference

Vps35^MH20/Vps35¹ has lethal - all die before end of prepupal stage phenotype, non-suppressible by Lrrk^e03680/Lrrk^e03680

(Inoshita et al., 2017)

Vps35^MH20/Vps35¹ has lethal - all die before end of prepupal stage phenotype, non-suppressible by Hsap\VPS35^UAS.cIa/Scer\GAL4^da.G32

(Inoshita et al., 2017)

Vps35^MH20/Vps35¹ has lethal - all die before end of prepupal stage phenotype, non-suppressible by Scer\GAL4^da.G32/Hsap\VPS35^{D620N.UAS.cIa}

(Inoshita et al., 2017)

Phenotype Manifest In

Suppressed by

Statement

Reference

Vps35^MH20/Vps35¹ has type I terminal bouton | third instar larval stage phenotype, suppressible by Rab5^UAS.GFP/Scer\GAL4^da.G32

(Inoshita et al., 2017)

Vps35^MH20/Vps35¹ has type I terminal bouton | third instar larval stage phenotype, suppressible by Rab11^{UAS.ORF.Tag:HA}/Scer\GAL4^da.G32

(Inoshita et al., 2017)

Vps35^MH20/Vps35¹ has type I terminal bouton | third instar larval stage phenotype, suppressible by Lrrk^UAS.cIa/Scer\GAL4^da.G32

(Inoshita et al., 2017)

Vps35^MH20/Vps35¹ has type I terminal bouton | third instar larval stage phenotype, suppressible by Lrrk[+]/Lrrk^e03680

(Inoshita et al., 2017)

Vps35^MH20/Vps35¹ has synaptic vesicle | third instar larval stage phenotype, suppressible by Rab5^UAS.GFP/Scer\GAL4^da.G32

(Inoshita et al., 2017)

Vps35^MH20/Vps35¹ has synaptic vesicle | third instar larval stage phenotype, suppressible by Rab11^{UAS.ORF.Tag:HA}/Scer\GAL4^da.G32

(Inoshita et al., 2017)

Vps35^MH20/Vps35¹ has synaptic vesicle | third instar larval stage phenotype, suppressible by Lrrk^UAS.cIa/Scer\GAL4^da.G32

(Inoshita et al., 2017)

Vps35^MH20/Vps35¹ has synaptic vesicle | third instar larval stage phenotype, suppressible by Lrrk[+]/Lrrk^e03680

(Inoshita et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The prepupal lethality of Vps35¹/Vps35^MH20 transheterozygotes is not rescued by Lrrk^e03680 homozygosity.

In the third instar larval neuromuscular junctions of Vps35¹/Vps35^MH20 transheterozygotes, their reciprocal increased size and decreased number of synaptic vesicles observed are suppressed either by Lrrk^e03680 heterozygosity or by the expression of Rab5^{Scer\UAS.T:Avic\GFP}, Rab11^{Scer\UAS.ORF.T:Ivir\HA1} or Lrrk[Scer\UAS.cIa] under the control of Scer\GAL4^da.G32; their increased number of type Ib boutons and reciprocal decreased bouton size are also suppressed by the expression of either Rab5^{Scer\UAS.T:Avic\GFP} or Rab11^{Scer\UAS.ORF.T:Ivir\HA1} under the control of Scer\GAL4^da.G32.

In Vps35¹ heterozygous adults, their decreased sleep bouts are suppressed by the expression of Rab5^{Scer\UAS.T:Avic\GFP}, Rab11^{Scer\UAS.ORF.T:Ivir\HA1} or Lrrk^Scer\UAS.cIa under the control of Scer\GAL4^ple.PF or by Lrrk^e03680 heterozygosity; Lrrk^e03680 heterozygosity even leads to a significant increase in sleep bouts, as compared to controls.

(Inoshita et al., 2017)

The elongated dorsal trunk phenotype of Vps35¹ Rab9⁵⁶/Vps35¹ Rab9¹⁹⁹ double mutants is the same as that seen in either single mutant.

(Dong et al., 2013)

Xenogenetic Interactions

Statement

Reference

The prepupal lethality of Vps35¹/Vps35^MH20 transheterozygotes is not rescued by the Scer\GAL4^da.G32-driven expression of Hsap\VPS35^Scer\UAS.cIa or Hsap\VPS35^{D620N.Scer\UAS.cIa}.

(Inoshita et al., 2017)

Complementation and Rescue Data

Rescued by

Vps35^MH20/Vps35¹ is rescued by Scer\GAL4^da.G32/Vps35^UAS.cIa

(Inoshita et al., 2017)

Partially rescued by

Vps35^MH20/Vps35¹ is partially rescued by Vps35^D647N.UAS/Scer\GAL4^da.G32

(Inoshita et al., 2017)

Comments

The prepupal lethality of Vps35¹/Vps35^MH20 transheterozygotes is rescued by the Scer\GAL4^da.G32-driven expression of Vps35^Scer\UAS.cIa, but not of Vps35^{D647N.Scer\UAS}.

In the third instar larval neuromuscular junction of Vps35¹/Vps35^MH20 transheterozygotes, the decreased size of synaptic boutons is rescued by the expression of either Vps35^Scer\UAS.cIa or Vps35^{D647N.Scer\UAS} under the control of Scer\GAL4^da.G32; the decreased pool of synaptic vesicles, the associated reciprocal increased number and decreased size of synaptic boutons, the decreased endocytosis and the subtle ultrastructural defects of the subsynaptic reticulum are rescued by the expression of Vps35^Scer\UAS.cIa, but not Vps35^{D647N.Scer\UAS}, under the control of Scer\GAL4^da.G32; the increased miniature excitatory junction potential amplitude and the decreased excitatory junction potential amplitude under high stimulation conditions are rescued by the expression of Vps35^Scer\UAS.cIa, but not Vps35^{D647N.Scer\UAS}, under the control of either Scer\GAL4^da.G32, Scer\GAL4^Toll-6-D42 or Scer\GAL4^Mhc.PK.

The decreased sleep bouts displayed by Vps35¹ heterozygous adults are rescued by the expression of either Vps35^Scer\UAS.cIa or Vps35^{D647N.Scer\UAS} under the control of Scer\GAL4^ple.PF; the rescue by Vps35^{D647N.Scer\UAS} even leads to a significant increase in sleep bouts, as compared to wild-type controls.

(Inoshita et al., 2017)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Dvps35¹

(Belenkaya et al., 2008)

Vps35¹

dVps35¹

(Inoshita et al., 2017)

vps35¹

(Li et al., 2018, Dong et al., 2013, Dong et al., 2013)

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

Open Close