The complete regulatory region of the e locus (this includes the first intron as well as upstream sequences) derived from the "U76" dark pigmentation extraction line (which contains a homozygous third chromosome from a Ugandan population sample) is fused upstream of GFP.
Expression in abdomen and thorax showed reduction in activity in U76 strain (the source of this allele) compared to U53 strain. In males, expression is restricted from the two posterior segments, abdominal segments 5 and 6.