FlyBase Allele Report: Hsap\SLC31A1[UAS.cHa]

General Information

Symbol

Hsap\SLC31A1^UAS.cHa

Species

H. sapiens

Name

Saccharomyces cerevisiae UAS construct a of Hua

FlyBase ID

FBal0244508

Feature type

allele

Associated gene

Hsap\SLC31A1

Associated Insertion(s)

Carried in Construct

M{UAS-hCTR1.H}

Key Links

Transgenic product class

wild_type

(Hua et al., 2010)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Hua et al., 2010)

Mutagen

in vitro construct

(Hua et al., 2010)

Progenitor genotype

Carried in construct

M{UAS-hCTR1.H}

Cytology

Description

UASt regulatory sequences drive expression of Hsap\SLC31A1 sequences.

(Hua et al., 2010)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Hsap\SLC31A1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

viable, with Scer\GAL4^Act5C.PI

(Hua et al., 2010)

Phenotype Manifest In

eye | conditional, with Scer\GAL4^GMR.PF

(Hua et al., 2010)

Detailed Description

Statement

Reference

Flies expressing Hsap\CTR1^Scer\UAS.cHa ubiquitously and constitutively under the control of Scer\GAL4^Act5C.PI survive perfectly well on normal food. Only with high concentrations of copper (250μM) does Hsap\CTR1^Scer\UAS.cHa expression reduce the survival rate.

Overexpression of Hsap\CTR1^Scer\UAS.cHa in the eye under the control of Scer\GAL4^GMR.PF has no effect on the eye or survival rates when the flies are fed on normal food and even when they are raised on 250μM copper. A slight rough eye phenotype can be found on flies raised on 500μM copper.

(Hua et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Scer\GAL4^Tub.PU/Hsap\SLC31A1^UAS.cHa is a suppressor of lethal | larval stage phenotype of Ctr1A²⁵

(Hua et al., 2010)

Scer\GAL4^Tub.PU/Hsap\SLC31A1^UAS.cHa is a suppressor of abnormal developmental rate | larval stage phenotype of Ctr1A²⁵

(Hua et al., 2010)

Phenotype Manifest In

Suppressed by

Statement

Reference

Hsap\SLC31A1^UAS.cHa, Scer\GAL4^GMR.PF has eye | conditional phenotype, suppressible by Hsap\CCS^UAS.cHa, Scer\GAL4^GMR.PF

(Hua et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Co-expression of Hsap\CCS^Scer\UAS.cHa with Hsap\CTR1^Scer\UAS.cHa in the eye under the control of Scer\GAL4^GMR.PF results in a stronger rough eye phenotype.

Overexpression of Hsap\CTR1^Scer\UAS.cHa under the control of Scer\GAL4^tub.PU suppresses the lethality associated with developmental arrest found in Ctr1A²⁵ mutants.

(Hua et al., 2010)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

Hsap\CTR1^{Scer\UAS.T:SV40}

Hsap\CTR1^Scer\UAS.cHa

Hsap\SLC31A1^Scer\UAS.cHa

Hsap\SLC31A1^UAS.cHa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Hua

Secondary FlyBase IDs

References (1)

Report Sections

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