Flies expressing Ube3a^JF03406 under the control of Scer\GAL4^{elav.Switch.PO} or Scer\GAL4^{Mef2.247.Switch} (and feeding with RU486 to induce expression via the GeneSwitch system) do not exhibit defective learning in an aversive conditioning assay, but do exhibit defects in a reversal-learning paradigm, with a third-odor preference assay showing an inability to forget the first aversive odor association after training with a second aversive odor, although the ability to learn new behavior patterns is normal, as compared to controls.

There is no significant effect on EJP or RMP at the third instar larval neuromuscular junction with expression of Ube3a^JF03406 driven by Scer\GAL4^c179, Scer\GAL4^how-24B, Scer\GAL4^Mef2.PU or Scer\GAL4^elav-C155.