FlyBase Allele Report: Dmel\sktl[βTub85D.EYFP]

General Information

Symbol

Dmel\sktl^{βTub85D.EYFP}

Species

D. melanogaster

Name

FlyBase ID

FBal0245268

Feature type

allele

Associated gene

Dmel\sktl

Associated Insertion(s)

Carried in Construct

P{βTub85D-sktl.YFP}

Also Known As

YFP-Sktl

Key Links

Transgenic product class

wild_type

(Wei et al., 2008)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Wei et al., 2008)

Mutagen

in vitro construct

(Wei et al., 2008)

Progenitor genotype

Carried in construct

P{βTub85D-sktl.YFP}

Cytology

Description

The spermatocyte-specific βTub85D promoter drives expression of sktl tagged at the N-terminus with EYFP.

(Wei et al., 2008)

Allele components

Component

Use(s)

Regulatory region(s)

βTub85D

Encoded product / tool

sktl

Tagged with

EYFP

yellow fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter or direct label

Stage

Tissue/Position (including subcellular localization)

Reference

spermatogenesis

spermatid cyst | restricted

(Fabian et al., 2010)

Additional Information

Statement

Reference

sktl protein is localized to the growing end of the spermatid cyst

(Fabian et al., 2010)

Marker for

Reflects expression of

sktl

(Fabian et al., 2010)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressor of

Statement

Reference

sktl^{βTub85D.EYFP} is a suppressor | partially of spermatid cyst phenotype of Styp\sopB^{ΔN29.βTub85D}

(Fabian et al., 2010)

sktl^{βTub85D.EYFP} is a suppressor of spermatid cyst phenotype of Styp\sopB^{ΔN29.βTub85D}

(Wei et al., 2008)

sktl^{βTub85D.EYFP} is a suppressor of elongation stage spermatid phenotype of Styp\sopB^{ΔN29.βTub85D}

(Wei et al., 2008)

sktl^{βTub85D.EYFP} is a suppressor | partially of microtubule phenotype of Styp\sopB^{ΔN29.βTub85D}

(Wei et al., 2008)

sktl^{βTub85D.EYFP} is a suppressor of axoneme phenotype of Styp\sopB^{ΔN29.βTub85D}

(Wei et al., 2008)

sktl^{βTub85D.EYFP} is a suppressor | partially of ciliary basal body phenotype of Styp\sopB^{ΔN29.βTub85D}

(Wei et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Bipolarity of P{βTub85D-SigD}R1 cysts is partially rescued by co-expression of sktl^{βTub85D.T:Avic\GFP-YFP}.

(Fabian et al., 2010)

Expression of sktl^{βTub85D.T:Avic\GFP-YFP} restores spermatid elongation in Styp\sopB^{ΔN29.βTub85D}-expressing testes. Axoneme assembly is rescued, and the stability of the ensheathed structure by the axial membrane is restored. Orientation of doublet microtubules is occasionally disrupted. The orientation of the basal body with respect to the nuclear envelope is largely restored, although not all basal bodies are associated with a nucleus.

(Wei et al., 2008)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

sktl^{βTub85D.EYFP}

sktl^{βTub85D.T:Avic\GFP-YFP}

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

Open Close