The 8 amino acids (RYILIACH) at the junction of the third transmembrane domain and the second intracellular loop of the protein which are deleted in the protein produced by Tre1sctt mutants have been mutated to RYAAAAAA.
Tre1RYAAAAAA rescues Tre1sctt
Tre1RYAAAAAA rescues the germ cell migration defects of embryos derived from Tre1sctt homozygous females.