The 8 amino acids (RYILIACH) at the junction of the third transmembrane domain and the second intracellular loop of the protein which are deleted in the protein produced by Tre1sctt mutants have been mutated to AYILIACH.
Tre1AYILIACH fails to rescue Tre1sctt
Tre1AYILIACH fails to rescue the germ cell migration defects of embryos derived from Tre1sctt homozygous females.