Flies expressing Hsap\TOR1A^{ΔFY.Scer\UAS} in muscles (under the control of Scer\GAL4^C57) or in neurons (Scer\GAL4^elav-C155) become severely incapacitated when exposed to high temperatures (38[o]C), whereas the majority of control flies remain active.

Larvae expressing Hsap\TOR1A^{ΔFY.Scer\UAS} under the control of Scer\GAL4^elav-C155 exhibit aberrant bouton morphologies: the number of type I synaptic boutons is similar to controls, but the boutons are bigger and have more abnormal protrusions. The average length of the boutons does not differ from controls but the average surface area, volume and number of T-shaped active zones per bouton are all increased.

Abnormal free dense bodies are observed at the presynaptic terminals of larvae of larvae expressing Hsap\TOR1A^{ΔFY.Scer\UAS} under the control of Scer\GAL4^elav-C155. In contrast to controls, the post-synaptic subsynaptic reticulum is interrupted by small clearings in ~20% of larvae. The average area of the synapse is significantly increase compared with the average area of control synapses. There are increased number of functional synapses (ones with T-shaped active zones) and silent synapses (synapses lacking T-shaped active zones).