FlyBase Allele Report: Dmel\cyc[VP16.UAS]

General Information

Symbol

Dmel\cyc^VP16.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0246364

Feature type

allele

Associated gene

Dmel\cyc

Associated Insertion(s)

Carried in Construct

P{UAS-cyc.VP16}

Also Known As

uas-cyc-vp16

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Kadener et al., 2008)

Progenitor genotype

Carried in construct

P{UAS-cyc.VP16}

Cytology

Description

UASt regulatory sequences drive expression of cyc (lacking UTR sequences) which is fused to the VP16 activation domain.

(Kadener et al., 2008)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

cyc

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor rhythm, with Scer\GAL4^Act.PU

(Kadener et al., 2008)

abnormal locomotor rhythm, with Scer\GAL4^elav-C155

(Kadener et al., 2008)

abnormal locomotor rhythm, with Scer\GAL4^P2.4.Pdf

(Kadener et al., 2008)

abnormal locomotor rhythm, with Scer\GAL4^tim.PE

(Kadener et al., 2008)

Phenotype Manifest In

Detailed Description

Statement

Reference

Flies expressing cyc^{Scer\UAS.T:Hsim\VP16} under the control of Scer\GAL4^tim.PE are robustly rhythmic in a circadian locomotor assay, with approximately 22 hour periods (approximately 2 hours shorter than those of controls).

Flies expressing cyc^{Scer\UAS.T:Hsim\VP16} under the control of either Scer\GAL4^P2.4.Pdf, Scer\GAL4^elav-C155 or Scer\GAL4^Act.PU are rhythmic in a circadian locomotor assay and the period is shorter than that of controls in each case.

(Kadener et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

cyc^VP16.UAS/Scer\GAL4^tim.PE is a suppressor of abnormal locomotor rhythm phenotype of Clk^ar

(Kadener et al., 2008)

Other

Statement

Reference

Dcr-1^RNAi.UAS.cUa, Scer\GAL4^tim.PE, cyc^VP16.UAS has abnormal locomotor rhythm phenotype

(Kadener et al., 2009)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of cyc^{Scer\UAS.T:Hsim\VP16} and Dcr-1^VDRC.cUa under the control of Scer\GAL4^tim.PE at 29[o]C results in adults in which the amplitude of the locomotor activity rhythms is dramatically reduced compared to controls.

(Kadener et al., 2009)

Expression of cyc^{Scer\UAS.T:Hsim\VP16} under the control of Scer\GAL4^tim.PE fails to rescue circadian locomotor activity rhythms in Clk^ar flies under constant darkness conditions, but in light-dark conditions most of the abnormal features of locomotor activity seen in Clk^ar flies are rescued (higher diurnal rather than night activity as well as the lights-on startle response is restored).

Expression of cyc^{Scer\UAS.T:Hsim\VP16} under the control of Scer\GAL4^elav-C155 in a per⁰¹, per^Scer\UAS.cYa background only shortens the circadian locomotor activity period by 20 minutes, in contrast to the shortening of 2 hours seen when cyc^{Scer\UAS.T:Hsim\VP16} is expressed under the control of Scer\GAL4^elav-C155 in a wild-type background.

(Kadener et al., 2008)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

cyc^{Scer\UAS.T:Hsim\VP16}

cyc^VP16.UAS

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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