FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Khc1-849.αTub67C.GFP
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General Information
Symbol
Dmel\Khc1-849.αTub67C.GFP
Species
D. melanogaster
Name
FlyBase ID
FBal0246619
Feature type
allele
Associated gene
Dmel\Khc
Associated Insertion(s)
Carried in Construct
P{αTub67C-Khc.1-849-GFP}
Key Links
Transgenic product class
inframe_deletion
(Loiseau et al., 2010)
Mutagen
Nature of the Allele
Transgenic product class
inframe_deletion
(Loiseau et al., 2010)
Mutagen
in vitro construct
(Loiseau et al., 2010)
Progenitor genotype
Carried in construct
P{αTub67C-Khc.1-849-GFP}
Cytology
Description

The αTub67C promoter drives expression of a mutant "tailless" form of Khc that is missing the last 125 amino acid residues and which is tagged with GFP.

(Loiseau et al., 2010)
Allele components
Component
Use(s)
Regulatory region(s)
αTub67C
Encoded product / tool
Khc
Tagged with
GFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Approximately 60% of Khc1-849.αTub67C.T:Avic\GFP mutants oocytes exhibit incorrect nucleus positioning, with the nucleus found separated from the anterior membrane by more than half a nucleus radius.

      The presence of Khc1-849.αTub67C.T:Avic\GFP rescues the absence of dorsal appendages found in Khc27 mutants in 48% of cases. Only 3.5% of these mutants have two fully formed dorsal appendages.

      The presence of Khc1-849.αTub67C.T:Avic\GFP fails to rescue osk mRNA transport but does rescue the dynein transport defects found in Khc27 mutants.

      (Williams et al., 2014)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescues

      Khc1-849.αTub67C.GFP partially rescues Khc27

      (Williams et al., 2014)
      Comments

      The presence of Khc1-849.αTub67C.T:Avic\GFP fails to rescue osk mRNA transport but does rescue the dynein transport defects found in Khc27 mutants.

      Approximately 60% of Khc1-849.αTub67C.T:Avic\GFP mutants oocytes exhibit incorrect nucleus positioning, with the nucleus found separated from the anterior membrane by more than half a nucleus radius.

      The presence of Khc1-849.αTub67C.T:Avic\GFP rescues the absence of dorsal appendages found in Khc27 mutants in 48% of cases. Only 3.5% of these mutants have two fully formed dorsal appendages.

      (Williams et al., 2014)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Khc1-849.αTub67C.GFP
      Khc1-849.αTub67C.T:Avic\GFP
      Name Synonyms
      Secondary FlyBase IDs
        References (2)