FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\kisLM27
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General Information
Symbol
Dmel\kisLM27
Species
D. melanogaster
Name
FlyBase ID
FBal0246953
Feature type
allele
Associated gene
Dmel\kis
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
ethyl methanesulfonate
(Melicharek et al., 2008)
Progenitor genotype
Cytology
Description

Point mutation (C3193T) in the transcript that encodes the long kis isoform; this is predicted to result in a premature stop codon (FlyBase curator comment: the mutation is reported as occurring at residue Q1064, but there is no Q residue at position 1064 in the reference sequence, instead the change that introduces the premature stop codon has been mapped to residue Q1065).

(Gervais et al., 2019)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
2L:238,995..238,995 [-]
Nucleotide change:

C238995T

Amino acid change:

Q1065term | kis-PA; Q1065term | kis-PC; Q1065term | kis-PD; Q1065term | kis-PE; Q1065term | kis-PF

Reported amino acid change:

Q1064term

Comment:

The mutation is reported to be at aa residue Q1064 (codon CAA) but there is no Gln at position 1064 in the reference sequence. It was mapped to Q1065.

(Gervais et al., 2019)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      kisLM27/kisLM27 somatic clones in a heterozygous background contain a greater overall number of cells and significantly more intestinal stem cells in adult flies compared to controls.

      (Gervais et al., 2019)

      kisLM27/kisk13416 third instar larvae do not show gross defects in muscle morphology. Muscle 6 area is normal and the number of boutons per muscle 6/7 neuromuscular junction (NMJ) is not significantly different from wild type. The total number of synaptic branches per muscle 6/7 NMJ is significantly increased compared to wild type.

      kisLM27/kisk13416 third instar larvae show a significant decrease in both evoked excitatory junctional current (eEJC) amplitude and in miniature EJC amplitude at the neuromuscular junction compared to controls. mEJC frequency is not significantly different from controls. Quantal content is significantly reduced.

      (Ghosh et al., 2014)

      Homozygous γ Kenyon neurons undergo proper dendritic pruning during pupal development, but they display defects in their axonal pruning and continue to display unpruned γ axons projecting into the α lobes 40% of the time in pupal brains.

      Cells in homozygous clones in the α, β and γ lobes of the mushroom body have normal dendrite morphology, but show axonal migration defects with axons crossing the midline (55% penetrance), cell position defects (36% penetrance) and defects in the morphology of the lobe (55% penetrance).

      Homozygous dorsal cluster neuron clones in the larva show a number of defects; 53% show abnormalities in position within the brain, 32% show defects in axon migration (the neurons fail to migrate to their proper contralateral targets) and 32% of cell bodies are not part of the larger cell body cluster.

      Homozygous adult dorsal cluster neurons do not show defects in dendrite formation. However, they show a severe reduction in the number of axons extending into the lobula and subsequently extending from the lobula into the medulla.

      Homozygous clones in the developing third instar larval retina show abnormal axonal migration into the developing brain, with photoreceptor axons extending beyond their normal targets and radiating out into the surrounding brain areas. These axons also show abnormal defasciculation of axonal bundles in the optic stalk. External eye morphology appears normal in adults containing homozygous clones in the retina.

      (Melicharek et al., 2010)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Enhancer of
      Statement
      Reference

      kis[+]/kisLM27 is an enhancer of eye | heat sensitive phenotype of Df(3R)p13/ato1090

      (Melicharek et al., 2008)

      kis[+]/kisLM27 is an enhancer of photoreceptor neuron | heat sensitive phenotype of Df(3R)p13/ato1090

      (Melicharek et al., 2008)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      A kisLM27 heterozygous background gives a dominant mild enhancement to the ato1090/Df(3R)p13 eye phenotype at 25[o]C.

      (Melicharek et al., 2008)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Fails to complement

      kis1

      (Melicharek et al., 2008)

      kisk13416

      (Melicharek et al., 2008)
      Rescued by

      kisLM27 is rescued by kisUAS.L.Tag:polyHis,Tag:FLAG/Scer\GAL4Tub.PU

      (Gervais et al., 2019)
      Comments

      Expressing kisUAS.L.Tag:polyHis,Tag:FLAG under the control of Scer\GAL4Tub.PU fully rescues the increased overall number of cells and of intestinal stem cells in somatic clones homozygous for kisLM27.

      (Gervais et al., 2019)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      References (9)