FlyBase Allele Report: Dmel\Raf[ΔN114.Hsp83]

General Information

Symbol

Dmel\Raf^ΔN114.Hsp83

Species

D. melanogaster

Name

FlyBase ID

FBal0247945

Feature type

allele

Associated gene

Dmel\Raf

Associated Insertion(s)

Carried in Construct

P{Hsp83-Raf.ΔN114}

Key Links

Transgenic product class

inframe_deletion

(Ding et al., 2010)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

inframe_deletion

(Ding et al., 2010)

Mutagen

in vitro construct

(Ding et al., 2010)

Progenitor genotype

Carried in construct

P{Hsp83-Raf.ΔN114}

Cytology

Description

An Hsp83 promoter drives expression of phl amino acid residues 115-739.

(Ding et al., 2010)

Allele components

Component

Use(s)

Regulatory region(s)

Hsp83

Encoded product / tool

Raf

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhancer of

Statement

Reference

phl[+]/Raf^ΔN114.Hsp83 is an enhancer of ventral denticle belt | maternal effect | heat sensitive phenotype of tor^11D

(Ding et al., 2010)

NOT Suppressor of

Statement

Reference

Raf^ΔN114.Hsp83 is a non-suppressor of abdominal 8 ventral denticle belt | maternal effect phenotype of tor^rv66

(Ding et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

The denticle belt phenotype seen in larvae derived from derived from tor^11D/+ females at 29[o]C is enhanced by phl^Hsp83.PD (penetrance is increased to 31.8%) and, to a lesser degree, by phl^ΔN114.Hsp83 (penetrance is increased to 17.1%).

phl^ΔN114.Hsp83 does not rescue abdominal segment 8 denticle belts in embryos derived from homozygous trk¹ females.

The lack of the abdominal segment 8 denticle belts seen in embryos derived from homozygous tor^rv66 females is not rescued by phl^ΔN114.Hsp83.

(Ding et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Raf^ΔN114.Hsp83 rescues Raf¹¹

(Ding et al., 2010)

Comments

phl^ΔN114.Hsp83 can rescue the posterior defects seen in embryos derived from derived from females containing homozygous phl¹¹ germline clones (rescue is seen if the level of expression from the transgene is equal or greater than the endogenous wild-type level of phl protein), but phl^ΔN114.Hsp83 is less active than phl^Hsp83.PD in this assay (the latter can rescue the posterior defects at much lower levels of expression).

(Ding et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Daf^ΔN114

(Ding et al., 2010)

Raf^ΔN114.Hsp83

phl^ΔN114.Hsp83

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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