Induction of Elp3^{dsRNA.Scer\UAS} at 29[o]C under the control of Scer\GAL4^da.G32 results in a significant reduction in fly viability. Defects in development are not observed until the late pupal stage, which is the stage at which the majority of lethality occurs.

Expression of Elp3^{dsRNA.Scer\UAS} at 29[o]C in the brain and CNS under the control of Scer\GAL4^60IIA does not affect fly viability. However, these flies appear far more active than control flies in that they exhibit continuous movement, including circling the top of the vial and rapid jumping. Elp3^{dsRNA.Scer\UAS}-Scer\GAL4^60IIA flies climb more rapidly than control flies. Scer\GAL4^elav-C155-driven expression results in the same hyperactivity phenotype. Elp3^{dsRNA.Scer\UAS}-expressing flies that climb to the top of the vials continuously circle the upper portion of the tube and often execute rapid high jumps back down below the 9cm mark of the vial side shortly after climbing past it. Elp3^{dsRNA.Scer\UAS}-expressing flies exhibit a significant increase in the total activity of the flies. Both male and female flies show a significantly greater distance moved per movement compared to control flies.

Female flies in which Elp3^{dsRNA.Scer\UAS} is expressed at 29[o]C in the brain and CNS under the control of Scer\GAL4^60IIA or Scer\GAL4^elav-C155 sleep significantly less than the control flies both during the day and nighttime periods. Additionally, female flies exhibit significantly shorter sleep bouts. Male flies do not have significantly different total sleep but do show a small but significant increase in bout duration.

Fifteen- and sixteen-day old female flies in which Elp3^{dsRNA.Scer\UAS} is expressed at 29[o]C in the brain and CNS under the control of Scer\GAL4^60IIA show a significant loss of total sleep and have significantly shorter sleep bouts. Sleep deprived flies show no significant differences in the amount of recovery sleep required compared to control flies.

There is a dramatic increase (182%) in the number of synaptic boutons in Elp3^{dsRNA.Scer\UAS}-Scer\GAL4^elav-C155 larvae compared to control flies. Both type I and Ib boutons are more numerous, although the increase is higher in type I boutons compared to type Ib. Satellite bouton budding is indistinguishable from control flies. However, there is an increase in axonal arbor area in terms of length and branching relative to muscle surface area in these flies compared to controls.