FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\mad2Δ
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General Information
Symbol
Dmel\mad2Δ
Species
D. melanogaster
Name
FlyBase ID
FBal0247990
Feature type
allele
Associated gene
Dmel\mad2
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
P-element activity
(Buffin et al., 2007)
Progenitor genotype
S6k07084
(Buffin et al., 2007)
Caused by aberration
Df(3)07084
Cytology
Description

Generated through imprecise excision of P{PZ}S6k07084 resulting in Df(3)07084 with a deletion of 3150bp, removing the last 180bp of mad2, all of gene CG5537 and the first 1120bp of S6k.

(Buffin et al., 2007)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
deletion
3L:5,809,162..5,812,311 [-]
Comment:

Deletion resulting from imprecise excision of P{PZ}S6k07084. Location deduced from description of mad2Delta as a deletion of 3150 base pairs, removing the last 180 base pairs of mad2, all of kri, and the first 1120 base pairs of S6k. Deletion mapped from breakpoint in mad2.

(Buffin et al., 2007)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  microcephaly
      CEA
      (Buffin et al., 2007)
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      ameliorates  primary autosomal recessive microcephaly 5
      modeled by aspE3
      (Buffin et al., 2007)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      mad2Δ dividing cells in third instar larval brains rarely exhibit aneuploidy and do not show any increase in apoptosis. However, the aneuploid cells are qualitatively different from those of wild-type, with almost all being polyploids, suggesting a rare complete failure of segregation.

      mad2Δ mutants do not show accumulation of mitotic cells in colchicine-treated larval brains.

      There are no obvious aberrations in chromosome behaviour in mad2Δ mutants compared to controls. Mitosis appears to function normally in mad2Δ cells although anaphase onset is accelerated in mad2Δ mutant neuroblasts. The time from nuclear envelope breakdown to the onset of CycB degradation is substantially reduced in mad2Δ mutants. The average time required to degrade spindle-associated CycB and initiate anaphase in mad2Δ cells is longer than the average time for achieving alignment of kinetochores on the metaphase place.

      (Buffin et al., 2007)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressor of
      Statement
      Reference

      mad2Δ is a suppressor of abnormal mitotic cell cycle | larval stage phenotype of aspE3

      (Buffin et al., 2007)
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      mad2Δ mutants suppress the high mitotic index seen in aspE3 brains.

      (Buffin et al., 2007)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      mad2Δ
      (Ghosh et al., 2015, Buffin et al., 2007)
      Name Synonyms
      Secondary FlyBase IDs
        References (2)