Open Close
General Information
Symbol
Hsap\APPAβ40.UAS.Tag:SS(nec)
Species
H. sapiens
Name
FlyBase ID
FBal0248068
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-Aβ40, Aβ40, Aβ40, Aβ1-40, Alz40, UAS-Aβ1-40
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of the Tag:SS(nec) secretion signal peptide fused to the Aβ40 peptide.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

At ages less than 3 weeks, the latency of evoked dorsal longitudinal flight muscle (DLM) and Tergotrochanter muscle (TTM) excitatory junctional potentials (EJPs) is about 0.8ms in flies expressing one copy of Hsap\APP1-40.Scer\UAS.T:SS-nec under the control of Scer\GAL4OK307 (compared to 1.2ms in controls). At ages over 3 weeks, the latencies of evoked DLM and TTM EJPs in flies expressing one copy of Hsap\APP1-40.Scer\UAS.T:SS-nec are slightly but not significantly increased, suggesting that ageing does not cause a significant slowdown of neurotransmission in wild-type flies and in flies expressing Hsap\APP1-40.Scer\UAS.T:SS-nec.

Expression of Hsap\APP1-40.Scer\UAS.T:SS-nec under the control of Scer\GAL4elav.PU does not significantly shorten lifespan compared to controls. No climbing defects are observed in these flies.

Flies expressing Hsap\APP1-42.Scer\UAS.T:SS-nec under the control of Scer\GAL4elav.PU do not show any defects in locomotor rhythm in LD (12 hours light, 12 hours dark). In DD (total darkness) no different from controls is seen at 5, 35 days or 50 days, but a substantial difference in rhythm is seen at 50 days.

Adult flies expressing Hsap\APP1-40.Scer\UAS.T:SS-nec under the control of Scer\GAL4elav-C155 show similar median lifespan as controls and their climbing ability is also normal.

Flies expressing Hsap\APP1-40.Scer\UAS.T:SS-nec under the control of Scer\GAL4elav.Switch.PO from 2 day post-eclosion onwards (expression induced by feeding flies RU486) do not exhibit any effect on lifespan.

Expression of Hsap\APP1-40.Scer\UAS.T:SS-nec under the control of Scer\GAL4shot-OK307 does not significantly change either the flight ability or the lifespan of adult flies. It does however slightly hasten the age-related decline in neurotransmission functionality: the success rate of eliciting excitatory junction potentials (EJP) in the dorsal longitudinal muscle (DLM) fibers in response to brain stimulation is lower compared to controls.

Flies expressing Hsap\APP1-40.Scer\UAS.T:nec under the control of Scer\GAL4unspecified have a median survival of about 30 days.

Flies expressing Hsap\APP1-40.Scer\UAS.T:nec under the control of Scer\GAL4elav-C155 have normal eyes.

Expressing one copy of Hsap\APP1-40.Scer\UAS.T:nec has no effect on survival.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference

Expression of Hsap\APP1-40.Scer\UAS.T:SS-nec under the control of Scer\GAL4elav.PU in a per01 mutant background does not significantly shorten lifespan compared to controls. Climbing ability is also unaffected.

Expression of Fer1HCHVDRC.cUa in flies expressing Hsap\APP1-40.Scer\UAS.T:nec under the control of Scer\GAL4elav-C155 reduces hatching efficiency.

Expression of Fer2LCHVDRC.cUa in flies expressing Hsap\APP1-40.Scer\UAS.T:nec under the control of Scer\GAL4elav-C155 does not reduce hatching efficiency.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
Reported As
Symbol Synonym
Hsap\APP1-40.Scer\UAS.T:SS-nec
Hsap\APP1-40.Scer\UAS.T:nec
Hsap\APPAβ40.Scer\UAS.T:SS-nec
Hsap\APPAβ40.UAS.Tag:SS(nec)
Name Synonyms
Secondary FlyBase IDs
    References (13)