At ages less than 3 weeks, the latency of evoked dorsal longitudinal flight muscle (DLM) and Tergotrochanter muscle (TTM) excitatory junctional potentials (EJPs) is about 0.8ms in flies expressing one copy of Hsap\APP1-40.Scer\UAS.T:SS-nec under the control of Scer\GAL4OK307 (compared to 1.2ms in controls). At ages over 3 weeks, the latencies of evoked DLM and TTM EJPs in flies expressing one copy of Hsap\APP1-40.Scer\UAS.T:SS-nec are slightly but not significantly increased, suggesting that ageing does not cause a significant slowdown of neurotransmission in wild-type flies and in flies expressing Hsap\APP1-40.Scer\UAS.T:SS-nec.
Expression of Hsap\APP1-40.Scer\UAS.T:SS-nec under the control of Scer\GAL4elav.PU does not significantly shorten lifespan compared to controls. No climbing defects are observed in these flies.
Flies expressing Hsap\APP1-42.Scer\UAS.T:SS-nec under the control of Scer\GAL4elav.PU do not show any defects in locomotor rhythm in LD (12 hours light, 12 hours dark). In DD (total darkness) no different from controls is seen at 5, 35 days or 50 days, but a substantial difference in rhythm is seen at 50 days.
Expression of Hsap\APP1-40.Scer\UAS.T:SS-nec under the control of Scer\GAL4shot-OK307 does not significantly change either the flight ability or the lifespan of adult flies. It does however slightly hasten the age-related decline in neurotransmission functionality: the success rate of eliciting excitatory junction potentials (EJP) in the dorsal longitudinal muscle (DLM) fibers in response to brain stimulation is lower compared to controls.