FlyBase Allele Report: Dmel\His3.3A[KO]

General Information

Symbol

Dmel\His3.3A^KO

Species

D. melanogaster

Name

FlyBase ID

FBal0248470

Feature type

allele

Associated gene

Dmel\His3.3A

Associated Insertion(s)

P{?'RS5}His3.3A^KO

Carried in Construct

Also Known As

His3.3A^-, Df(2L)His3.3A

Key Links

Allele class

amorphic allele - molecular evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Hödl and Basler, 2009)

Mutagen

P-element activity

(Hödl and Basler, 2009)

Progenitor genotype

P{RS5}His3.3A^5-HA-1043

(Hödl and Basler, 2009)

Associated Insertion(s)

P{?'RS5}His3.3A^KO

Cytology

Description

Imprecise excision of the progenitor insertion, resulting in a deletion of the His3.3A coding sequence. 300bp of the left flank of the original insertion remain, which are joined to the 3'UTR of the downstream CG31918 gene.

(Hödl and Basler, 2009)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

His3.3A^KO, His3.3B^KO has sterile phenotype, suppressible by His3^His3.3B.PH

(Hödl and Basler, 2012)

Other

Statement

Reference

His3.3A^KO, His3.3B^KO has viable phenotype

(Hödl and Basler, 2009)

His3.3A^KO, His3.3B^KO has male sterile phenotype

(Hödl and Basler, 2009)

His3.3A^KO, His3.3B^KO has female sterile phenotype

(Hödl and Basler, 2009)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

His3^His3.3B.PH rescues the infertility of His3.3A^KO His3.3B^KO double mutants, and the flies can be maintained as a permanent stock.

Clones triply homozygous for His3.3A^KO, His3.3B^KO and Df(2L)BSC104 survive if they are carrying both His3.3B^K4R and two copies of M{3xHisGU.H3.2.K4R}, although the clones are smaller than their wild-type twin spots.

Clones triply homozygous for His3.3A^KO, His3.3B^KO and Df(2L)BSC104 survive if they are carrying both His3.3B^K4A and two copies of M{3xHisGU.H3.2.K4A}, although the clones are smaller than their wild-type twin spots.

(Hödl and Basler, 2012)

His3.3A^KO His3.3B^KO double homozygotes are sterile in both sexes but are viable to the adult stage with no obvious morphological defects.

The rare offspring of females containing His3.3A^KO His3.3B^KO double homozygous germline clones are phenotypically normal, irrespective of whether they paternally inherit His3.3+ function or not.

His3.3B^Tag:HA, His3.3B^K9A.Tag:HA and His3.3B^cHa each rescue the sterility of His3.3A^KO His3.3B^KO double homozygous males.

His3.3B^K4A.Tag:HA does not rescue the sterility of His3.3A^KO His3.3B^KO double homozygous males.

His3.3B^H3.2.Tag:HA does not rescue the sterility of His3.3A^KO His3.3B^KO double homozygous males.

(Hödl and Basler, 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

98089

P{hsp70-FLP}1, w^* P+PBac{XP3.RB5}His3.3B^KO; His3.3A^KO/SM6b

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Df(2L)His3.3A

(Faragó et al., 2022, Dorafshan et al., 2019)

His3.3A^-

(Hödl and Basler, 2012, Hödl and Basler, 2009)

His3.3A^KO

(Faragó et al., 2022, Song et al., 2018)

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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