FlyBase Allele Report: Dmel\His3.3B[KO]

Recombination between the two progenitor insertions results in the loss of the complete coding sequence of His3.3B (and the loss of the entire CG11042 transcription unit).

(Hödl and Basler, 2009)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

His3.3A^KO, His3.3B^KO has sterile phenotype, suppressible by His3^His3.3B.PH

(Hödl and Basler, 2012)

Suppressor of

Statement

Reference

His3.3B^KO/His3.3B^KO is a suppressor | partially of lethal - all die before end of pupal stage | recessive phenotype of BRWD3^PX2

(Chen et al., 2015)

Other

Statement

Reference

His3.3A^KO, His3.3B^KO has viable phenotype

(Hödl and Basler, 2009)

His3.3A^KO, His3.3B^KO has male sterile phenotype

(Hödl and Basler, 2009)

His3.3A^KO, His3.3B^KO has female sterile phenotype

(Hödl and Basler, 2009)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

His3^His3.3B.PH rescues the infertility of His3.3A^KO His3.3B^KO double mutants, and the flies can be maintained as a permanent stock.

Clones triply homozygous for His3.3A^KO, His3.3B^KO and Df(2L)BSC104 survive if they are carrying both His3.3B^K4R and two copies of M{3xHisGU.H3.2.K4R}, although the clones are smaller than their wild-type twin spots.

Clones triply homozygous for His3.3A^KO, His3.3B^KO and Df(2L)BSC104 survive if they are carrying both His3.3B^K4A and two copies of M{3xHisGU.H3.2.K4A}, although the clones are smaller than their wild-type twin spots.

(Hödl and Basler, 2012)

His3.3A^KO His3.3B^KO double homozygotes are sterile in both sexes but are viable to the adult stage with no obvious morphological defects.

The rare offspring of females containing His3.3A^KO His3.3B^KO double homozygous germline clones are phenotypically normal, irrespective of whether they paternally inherit His3.3+ function or not.

His3.3B^Tag:HA, His3.3B^K9A.Tag:HA and His3.3B^cHa each rescue the sterility of His3.3A^KO His3.3B^KO double homozygous males.

His3.3B^K4A.Tag:HA does not rescue the sterility of His3.3A^KO His3.3B^KO double homozygous males.

His3.3B^H3.2.Tag:HA does not rescue the sterility of His3.3A^KO His3.3B^KO double homozygous males.

(Hödl and Basler, 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

98089

P{hsp70-FLP}1, w^* P+PBac{XP3.RB5}His3.3B^KO; His3.3A^KO/SM6b

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

His3.3B^-

(Chen et al., 2015, Hödl and Basler, 2012, Hödl and Basler, 2009)

His3.3B^KO

ΔHis3.3B

(Dorafshan et al., 2019)

Name Synonyms

Secondary FlyBase IDs

FBab0046937

References (4)

Report Sections

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