FlyBase Allele Report: Dmel\pyd[ex180]

General Information

Symbol

Dmel\pyd^ex180

Species

D. melanogaster

Name

FlyBase ID

FBal0248976

Feature type

allele

Associated gene

Dmel\pyd

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Djiane et al., 2011)

Progenitor genotype

P{GawB}pyd^NP4400

(Djiane et al., 2011)

Cytology

Description

Imprecise excision of the progenitor insertion, P{GawB}pyd^NP4400, results in a deletion encompassing a large region of pyd.

(Djiane et al., 2011)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

fertile

(Djiane et al., 2011)

increased cell number (with Df(3R)p-XT103)

(Djiane et al., 2011)

some die during embryonic stage

(Choi et al., 2011)

some die during embryonic stage (with pyd^B12)

(Choi et al., 2011)

viable

(Djiane et al., 2011)

visible (with Df(3R)p-XT103)

(Djiane et al., 2011)

Phenotype Manifest In

dorsocentral bristle | increased number (with Df(3R)p-XT103)

(Djiane et al., 2011)

embryo | dorsal closure stage

(Choi et al., 2011)

embryo | dorsal closure stage (with pyd^B12)

(Choi et al., 2011)

embryonic head

(Choi et al., 2011)

embryonic head (with pyd^B12)

(Choi et al., 2011)

embryonic leading edge cell

(Choi et al., 2011)

embryonic leading edge cell (with pyd^B12)

(Choi et al., 2011)

ventral head epidermis

(Choi et al., 2011)

ventral head epidermis (with pyd^B12)

(Choi et al., 2011)

wing (with Df(3R)p-XT103)

(Djiane et al., 2011)

Detailed Description

Statement

Reference

Embryos maternally and zygotically mutant for pyd^ex180 exhibit 66% embryonic lethality.

Substantial embryonic lethality (43-58%) is found in flies from pyd^ex180 mothers crossed with pyd^B12 fathers.

The ventral epidermis is intact in pyd^ex180 maternal/zygotic mutant embryos and the CNS is not obviously hypertrophied. While approximately 17% of pyd^ex180 maternal/zygotic mutant embryos appear wild-type, most mutant embryos (32%) exhibit defects in head involution that result in defective head skeletons, whereas the most severe (51%) display complete failure of head involution, leading to a hole in the cuticle. In addition, some show minor defects in dorsal closure.

The ventral epidermis is intact in pyd^B12/pyd^ex180 maternal/zygotic mutant embryos and the CNS is not obviously hypertrophied. While approximately 12% of pyd^B12/pyd^ex180 maternal/zygotic mutant embryos appear wild-type, most mutant embryos (54%) exhibit defects in head involution that result in defective head skeletons, whereas the most severe (34%) display complete failure of head involution, leading to a hole in the cuticle. In addition, some show minor defects in dorsal closure.

(Choi et al., 2011)

Over 90% of pyd^ex180/Df(3R)p-XT103 flies display more than four dorsocentral macrochaetae.

Wings from pyd^ex180/Df(3R)p-XT103 flies are broader compared to control wings. A 10% increase in the number of cells found between the L3 and L4 veins is observed.

(Djiane et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

pyd^ex180/pyd[+] is an enhancer of abnormal cell shape phenotype of ena²³

(Choi et al., 2011)

Phenotype Manifest In

Enhanced by

Statement

Reference

pyd^ex180 has embryo | dorsal closure stage phenotype, enhanceable by ena²³/ena[+]

(Choi et al., 2011)

pyd^ex180 has embryonic leading edge cell phenotype, enhanceable by ena²³/ena[+]

(Choi et al., 2011)

pyd^ex180 has ventral head epidermis phenotype, enhanceable by ena²³/ena[+]

(Choi et al., 2011)

pyd^ex180 has embryonic head phenotype, enhanceable by ena²³/ena[+]

(Choi et al., 2011)

Enhancer of

Statement

Reference

pyd^ex180/pyd[+] is an enhancer of embryo | dorsal closure stage phenotype of ena²³

(Choi et al., 2011)

pyd^ex180/pyd[+] is an enhancer of embryonic leading edge cell phenotype of ena²³

(Choi et al., 2011)

pyd^ex180/pyd[+] is an enhancer of ventral head epidermis phenotype of ena²³

(Choi et al., 2011)

pyd^ex180/pyd[+] is an enhancer of embryonic head phenotype of ena²³

(Choi et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Reducing ena levels, through an ena²³ heterozygous background, substantially enhances the severity of the pyd^ex180 zygotic/maternal phenotype; more than one-third of the mutants exhibit large holes in the cuticle, suggesting disruption of epithelial integrity.

Reducing pyd levels, through a pyd^ex180 heterozygous background, strongly enhances the morphogenesis defects found in zygotic ena²³ mutants, substantially increasing the frequency of failure of head involution.

(Choi et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

pyd¹⁸⁰

(Shimizu et al., 2017)

pyd^ex180

(Choi et al., 2011, Djiane et al., 2011)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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