FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Hsap\LRRK2UAS.Tag:FLAG
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General Information
Symbol
Hsap\LRRK2UAS.Tag:FLAG
Species
H. sapiens
Name
FlyBase ID
FBal0249096
Feature type
allele
Associated gene
Hsap\LRRK2
Associated Insertion(s)
Carried in Construct
P{UAS-LRRK2.FLAG}
Also Known As
UAS-LRRK2, UAS-hLRRK2, UAS-WT-LRRK2, UAS-hLRRK2-WT-Flag
Key Links
Transgenic product class
wild_type
(Liu et al., 2008)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Liu et al., 2008)
Mutagen
in vitro construct
(Liu et al., 2008)
Progenitor genotype
Carried in construct
P{UAS-LRRK2.FLAG}
Cytology
Description

UASt regulatory sequences drive expression of Hsap\LRRK2 with an N-terminal Tag:FLAG-tag.

(Liu et al., 2008)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Hsap\LRRK2
Tagged with
Tag:FLAG
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      model of  Parkinson's disease
      is ameliorated by Hsap\ARFGAP1Δ64.UAS.Tag:HA
      (Xiong et al., 2012)
      is ameliorated by Hsap\ARFGAP1UAS.Tag:HA
      (Xiong et al., 2012)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of Hsap\LRRK2Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4ple.PF does not strongly affect the reduced contrast response steady-state visual evoked potentials method in 7-day-old adult flies.

      (Mortiboys et al., 2015)

      Flies expressing Scer\GAL4VGlut-OK371> mutants show neuronal responses to visual stimuli as reflected in frequency-tagged steady-state visually evoked potentials similar to controls.

      (Afsari et al., 2014)

      Axonal transport of mitochondria is not affected in larval motor neurons expressing Hsap\LRRK2Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4D42.

      Climbing and flight ability are normal in flies expressing Hsap\LRRK2Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4D42.

      (Godena et al., 2014)

      Flies expressing Hsap\LRRK2Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4ple.PG do not show a progressive reduction in electroretinogram amplitude (there is no significant difference in the amplitude seen in 3 and 28 day old flies). The retina, lamina and medulla of 28 day old flies do not show neurodegeneration defects and the external surface of the eye appears normal. Photoreceptor mitochondria do not show defects.

      Flies expressing Hsap\LRRK2Scer\UAS.T:Zzzz\FLAG under the control of either Scer\GAL4GMR.long or Scer\GAL4Act5C.PU show a significant reduction in ERG amplitude at 28 days of age compared to 3 days of age.

      Flies expressing Hsap\LRRK2Scer\UAS.T:Zzzz\FLAG under the control of either Scer\GAL4ple.PG, Scer\GAL4Ddc.HL9, Scer\GAL4elav.PLu, Scer\GAL4nSyb.PG or Scer\GAL4NP5214 show no significant difference in ERG amplitude at 3 and 28 days of age.

      (Hindle et al., 2013)

      Overexpression of Hsap\LRRK2Scer\UAS.T:Zzzz\FLAG in dopaminergic neurons, under the control of Scer\GAL4ple.PF, leads to a substantial reduction of dopamine neurons in the adult brain hemisphere.

      Expression of Hsap\LRRK2Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4ple.PF significantly reduces climbing ability.

      (Xiong et al., 2012)

      Treatment of flies expressing Hsap\LRRK2Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4Ddc.PL with 0.1% H[[2]]O[[2]] significantly decreases survival, compared with control flies and untreated Hsap\LRRK2Scer\UAS.T:Zzzz\FLAG-Scer\GAL4Ddc.PL flies. Treatment with GW5074 does not affect the survival of Hsap\LRRK2 transgenic flies exposed to 0.1% H[[2]]O[[2]].

      The addition of 1mM curcumin to the food given to Scer\GAL4Ddc.PL->Hsap\LRRK2Scer\UAS.T:Zzzz\FLAG flies with or without 0.1% H[[2]]O[[2]] from day 1 post-eclosion results in an increase in survival. Curcumin has no effect on non-transgenic control flies.

      The addition of 0.1% H[[2]]O[[2]] from day 1 post-eclosion onwards significantly worsens the locomotor impairment seen in flies expressing Hsap\LRRK2Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4Ddc.PL, compared with untreated transgenic flies. Treatment with 5υM GW5074 or 1mM curcumin improves but does not fully reverse the locomotor impairment seen in these flies.

      Treatment of flies expressing Hsap\LRRK2Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4Ddc.PL with 0.1% H[[2]]O[[2]] induces loss of DA neurons. Treatment with 5υM GW5074 or 1mM curcumin in the food from day 1 post-eclosion throughout the lifetime of Scer\GAL4Ddc.PL-->Hsap\LRRK2G2019S.Scer\UAS.T:Zzzz\FLAG suppresses the loss of ple-positive neurons.

      (Yang et al., 2012)

      Overexpression of Hsap\LRRK2Scer\UAS.T:Zzzz\FLAG pre- or post-synaptically (under the control of Scer\GAL4elav-C155 or Scer\GAL4Mhc.PW, respectively) results in a reduction in the total number of type I boutons on muscle 6/7 from the A3 segment. In addition, neuromuscular junction length and branch number are decreased by 25%. Separate quantification of type Ib versus type Is boutons on muscle 6/7 reveals a similar degree (20%) of decrease in the number of each type of bouton.

      The neuromuscular junction morphology phenotype caused by overexpression of Hsap\LRRK2Scer\UAS.T:Zzzz\FLAG by Scer\GAL4Mhc.PW is rescued by rapamycin treatment.

      Scer\GAL4Mhc.PW-driven expression of Hsap\LRRK2Scer\UAS.T:Zzzz\FLAG does not affect neuromuscular junction MT cytoskeleton formation.

      Newly synthesized tubulin inside synaptic boutons is more fragmented in animals expressing Hsap\LRRK2Scer\UAS.T:Zzzz\FLAG presynaptically (under the control of Scer\GAL4elav-C155).

      Neuronal overexpression of Hsap\LRRK2Scer\UAS.T:Zzzz\FLAG (under the control of Scer\GAL4elav-C155) leads to a decrease of mitochondria number in terminal boutons and a significant increase of mEJC frequency. Quantal content is not significantly changed in these animals compared to controls.

      (Lee et al., 2010)

      Expression of Hsap\LRRK2Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4GMR.PF results in retinal degeneration by 3 weeks after eclosion.

      Expression of Hsap\LRRK2Scer\UAS.T:Zzzz\FLAG driven by Scer\GAL4Ddc.PL causes early mortality and locomotion impairment.

      Expression of Hsap\LRRK2Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4Ddc.PL leads to selective loss of dopaminergic neurons.

      Expression of Hsap\LRRK2Scer\UAS.T:Zzzz\FLAG driven by Scer\GAL4elav.PU causes late-onset locomotion impairment, shortened lifespan and selective loss of dopaminergic neurons.

      (Liu et al., 2008)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Suppressed by
      NOT suppressed by
      Suppressor of
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference

      Co-expression of Hsap\ARFGAP1Scer\UAS.T:Ivir\HA1 with Hsap\LRRK2Scer\UAS.T:Zzzz\FLAG in dopaminergic neurons, under the control of Scer\GAL4ple.PF, significantly prevents the loss of dopamine neurons in PAL and PPM1/2 but not PPM3 or PPL1 clusters.

      Co-expression of Hsap\ARFGAP1Δ64.Scer\UAS.T:Ivir\HA1 with Hsap\LRRK2Scer\UAS.T:Zzzz\FLAG in dopaminergic neurons, under the control of Scer\GAL4ple.PF, rescues dopaminergic neuronal loss in the PPM1/2 but does not significantly rescue in the other dopaminergic clusters.

      Co-expression of either Hsap\ARFGAP1Scer\UAS.T:Ivir\HA1 or Hsap\ARFGAP1Δ64.Scer\UAS.T:Ivir\HA1 in dopaminergic neurons, under the control of Scer\GAL4ple.PF, rescues the climbing defect seen in Hsap\LRRK2Scer\UAS.T:Zzzz\FLAG-expressing flies.

      Co-expression of Hsap\LRRK2Scer\UAS.T:Zzzz\FLAG rescues the eye defects seen upon expression of Hsap\ARFGAP1Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4GMR.PF.

      (Xiong et al., 2012)

      Co-expression of futschEP1419 pre-synaptically rescues the synaptic overgrowth phenotype found upon expression of Hsap\LRRK2Scer\UAS.T:Zzzz\FLAG pre-synaptically under the control of Scer\GAL4elav-C155. However, synaptic defects caused by post-synaptic expression of Hsap\LRRK2Scer\UAS.T:Zzzz\FLAG are not suppressed when futschEP1419 is expressed post-synaptically (under the control of Scer\GAL4Mhc.PW).

      The abnormal microtubule phenotype seen upon Hsap\LRRK2Scer\UAS.T:Zzzz\FLAG presynaptic expression is rescued by a futschEP1419 expression (both under the control of Scer\GAL4elav-C155).

      Neuronal co-expression of futschEP1419 with Hsap\LRRK2Scer\UAS.T:Zzzz\FLAG rescues the mitochondrial distribution phenotype seen upon Hsap\LRRK2Scer\UAS.T:Zzzz\FLAG expression alone.

      (Lee et al., 2010)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Hsap\LRRK2Scer\UAS.T:Zzzz\FLAG
      Hsap\LRRK2UAS.Tag:FLAG
      hLRRK2
      (Mortiboys et al., 2015)
      Name Synonyms
      Secondary FlyBase IDs
        References (18)