UAS regulatory sequences drive expression of wild-type Ir8a with the endogenous signal sequence replaced with Tag:SS(hCALR) and fused to mCherry.
Neurons co-expressing Ir84aScer\UAS.T:Hsap\CALR,T:Avic\GFP-EGFP and Ir8aScer\UAS.T:Hsap\CALR,T:Disc\RFP-mCherry under the control of Scer\GAL4Or22a.7.717 respond to phenylacetaldehyde in a concentration-dependent manner.