FlyBase Allele Report: Dmel\ImpL2[Def20]

General Information

Symbol

Dmel\ImpL2^Def20

Species

D. melanogaster

Name

FlyBase ID

FBal0249379

Feature type

allele

Associated gene

Dmel\ImpL2

Associated Insertion(s)

Carried in Construct

Also Known As

Imp-L2^Def20

Key Links

Allele class

amorphic allele - molecular evidence

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Honegger et al., 2008)

Mutagen

Delta2-3 transposase

(Honegger et al., 2008)

Progenitor genotype

P{}ImpL2^GE24013

(Honegger et al., 2008)

Cytology

Description

Imprecise excision of P{}ImpL2^GE24013 resulting in the deletion of the entire ImpL2 coding sequence.

(Honegger et al., 2008)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal stress response | third instar larval stage (with ImpL2^Def42)

(Honegger et al., 2008)

hyperplasia (with ImpL2^Def42)

(Honegger et al., 2008)

increased body size (with ImpL2^Def42)

(Honegger et al., 2008)

viable (with ImpL2^Def42)

(Honegger et al., 2008)

Phenotype Manifest In

ovary (with ImpL2^Def42)

(Honegger et al., 2008)

Detailed Description

Statement

Reference

ImpL2^Def20 mutant flies exhibit a reduction in circulating trehalose concentration. Whole-body trehalose levels are similar to controls.

(Kwon et al., 2015)

The body size of ImpL2^Def20/ImpL2^Def42 mutants is increased relative to wild-type flies. The size increase is attributable primarily to an increase in the number of cells, because cell size is only slightly affected. Apart from the size and weight increase, ImpL2^Def20/ImpL2^Def42 flies appear completely normal and eclose at the expected frequency and are not delayed in development. The weight increase is more pronounced in ImpL2^Def20/ImpL2^Def42 females than in males, although the increases in wing area and cell number are similar. This differential effect is caused by enlarged ovaries.

Survival of ImpL2^Def20/ImpL2^Def42 early third instar larvae is severely compromised under starvation conditions.

(Honegger et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

ImpL2^Def20/ImpL2^Def42 has viable phenotype, suppressible by Scer\GAL4^arm.PS/Ilp2^UAS.cBa

(Honegger et al., 2008)

Phenotype Manifest In

Suppressor of

Statement

Reference

ImpL2^Def20 is a suppressor of ovary phenotype of Scer\GAL4^NP5130, yki^{S111A.S168A.S250A.UAS.Tag:V5}

(Kwon et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

ImpL2^Def20 suppresses the increase in circulating trehalose concentration and whole-body trehalose levels seen in adult intestinal stem cells expressing yki^{S111A.S168A.S250A.Scer\UAS.T:SV5\V5} (under the control of Scer\GAL4^esg-NP5130 and limited to the adult stage using Scer\GAL80^ts.αTub84B).

The wasting of ovaries seen in flies expressing yki^{S111A.S168A.S250A.Scer\UAS.T:SV5\V5} (under the control of Scer\GAL4^esg-NP5130 and limited to the adult stage using Scer\GAL80^ts.αTub84B) is suppressed by ImpL2^Def20.

The reduction in glucose levels seen in the body void (excluding the midgut and hemolymph) of flies expressing yki^{S111A.S168A.S250A.Scer\UAS.T:SV5\V5} (under the control of Scer\GAL4^esg-NP5130 and limited to the adult stage using Scer\GAL80^ts.αTub84B) is suppressed by ImpL2^Def20.

(Kwon et al., 2015)

Overexpression of Ilp2^Scer\UAS.cBa under the control of Scer\GAL4^arm.PS causes lethality in ImpL2^Def42/ImpL2^Def20 mutants.

(Honegger et al., 2008)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

ImpL2^Def20/ImpL2^Def42 is rescued by ImpL2^+t9

(Honegger et al., 2008)

Comments

ImpL2^+t9 rescues the increased weight phenotype of ImpL2^Def20/ImpL2^Def42 mutants.

(Honegger et al., 2008)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

Def20

(Owusu-Ansah et al., 2013)

Imp-L2 def20

(Sarraf-Zadeh et al., 2013)

Imp-L2-def20

(Bader et al., 2013)

Imp-L2^Def20

(Banzai and Nishimura, 2023, Honegger et al., 2008)

ImpL2^Def20

(Han et al., 2026, Figueroa-Clarevega and Bilder, 2015, Kwon et al., 2015)

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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