FB2021_02, released April 13, 2021

Allele: Dmel\Gr5aUAS.cDa

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General Information
Symbol
Dmel\Gr5aUAS.cDa
Species
D. melanogaster
Name
Gustatory receptor 5a promoter construct of Dahanukar
FlyBase ID
FBal0256891
Feature type
allele
Associated gene
Dmel\Gr5a
Associated Insertion(s)
Carried in Construct
P{UAS-Gr5a.D}
Also Known As
UAS-Gr5a
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
in vitro construct - regulatory fusion
(Dahanukar et al., 2007)
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
       
      Progenitor genotype
      Carried in construct
      P{UAS-Gr5a.D}
      Cytology
      Nature of the lesion
      Statement
      Reference

      UAS regulatory sequences drive expression of a full-length Gr5a cDNA.

      (Dahanukar et al., 2007)
      Allele components
      Product class / Tool use(s)
      Regulatory region(s)
      UAS
      Encoded product / tool
      Gr5a
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of Gr5aScer\UAS.cDa in ab1C neurons, under the control of Scer\GAL4Gr21a.9.323 or Scer\GAL4Gr63a.PF, is sufficient to confer trehalose response in the ab1C neuron. Application of sucrose does not alter the firing rate of ab1C neuron expressing Gr5a. These neurons respond to application of glucose, m-glucoside, and melezitose.

      (Freeman et al., 2014)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      A Gr63a1 mutant background does not alter the response to trehalose, glucose, m-glucoside, or melezitose in flies expressing Gr5aScer\UAS.cDa in ab1C neurons, under the control of Scer\GAL4Gr21a.9.323 or Scer\GAL4Gr63a.PF.

      Co-expression of Gr64fScer\UAS.cFa, Gr64eScer\UAS.cFa, Gr61aScer\UAS.cFa, Gr64bScer\UAS.cJa, Gr64cScer\UAS.cFa, or Gr64dScer\UAS.cFa does not result in any significant alteration in the response to trehalose, m-glucoside, melezitose and glucose, in flies expressing Gr5aScer\UAS.cDa in ab1C neurons, under the control of Scer\GAL4Gr21a.9.323 or Scer\GAL4Gr63a.PF.

      Co-expression of Gr61aScer\UAS.cFa does not result in any significant alteration in the response to trehalose, m-glucoside, and melezitose, in flies expressing Gr5aScer\UAS.cDa in ab1C neurons, under the control of Scer\GAL4Gr21a.9.323 or Scer\GAL4Gr63a.PF.

      (Freeman et al., 2014)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Gr5aUAS.cDa/Scer\GAL4Gr5a.8.5 rescues Gr5aΔEP5

      (Dahanukar et al., 2007)
      Comments

      Expression of Gr5aScer\UAS.cDa under the control of Scer\GAL4Gr5a.8.5 rescues the reduction in the electrophysiological response to the sugars trehalose, m-α-glucoside, glucose and melezitose that is seen in Gr5aΔEP5 flies.

      Expression of Gr5aScer\UAS.cDa under the control of Scer\GAL4Gr5a.8.5 increases the mean proboscis extension response to all sugars whose responses are reduced in Gr5aΔEP5 flies.

      (Dahanukar et al., 2007)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Gr5aScer\UAS.cDa
      Gr5aUAS.cDa
      Name Synonyms
      Gustatory receptor 5a promoter construct of Dahanukar
      Secondary FlyBase IDs
        References (3)