FlyBase Allele Report: Dmel\cnn[Δ1.UASp.EGFP]

General Information

Symbol

Dmel\cnn^{Δ1.UASp.EGFP}

Species

D. melanogaster

Name

FlyBase ID

FBal0256921

Feature type

allele

Associated gene

Dmel\cnn

Associated Insertion(s)

Carried in Construct

P{UASp-cnn.Δ1}

Key Links

Transgenic product class

inframe_deletion

(Zhang and Megraw, 2007)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

inframe_deletion

(Zhang and Megraw, 2007)

Mutagen

in vitro construct

(Zhang and Megraw, 2007)

Progenitor genotype

Carried in construct

P{UASp-cnn.Δ1}

Cytology

Description

UASp regulatory sequences drive expression of a cnn coding sequence which lacks the amino terminal 17 amino acids of the maternal cnn-RA transcript and in which 13 core amino acid residues in the conserved "motif 1" have been deleted. The cnn sequences are tagged with EGFP.

(Zhang and Megraw, 2007)

Allele components

Component

Use(s)

Regulatory region(s)

UASp

binary expression system - regulatory region

Encoded product / tool

cnn

Tagged with

EGFP

green fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die during embryonic stage | maternal effect, with Scer\GAL4^{VP16.nanos.UTR}

(Zhang and Megraw, 2007)

some die during embryonic stage | maternal effect, with Scer\GAL4^{VP16.nanos.UTR}

(Zhang and Megraw, 2007)

Phenotype Manifest In

centriolar satellite, with Scer\GAL4^{VP16.nanos.UTR}

(Zhang and Megraw, 2007)

centrosome, with Scer\GAL4^{VP16.nanos.UTR}

(Zhang and Megraw, 2007)

Detailed Description

Statement

Reference

Embryos expressing cnn^{Δ1.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} do not hatch. Centrosome duplication appears normal in these embryos, but centrosome separation fails. The mutant embryos also have reduced numbers of centrosomal satellites.

Expression of cnn^{Δ1.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^tub.PU from late embryogenesis onwards is not zygotically lethal.

(Zhang and Megraw, 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to rescue

cnn^{Δ1.UASp.EGFP}/Scer\GAL4^{VP16.nanos.UTR} fails to rescue cnn^HK21/cnn^25cn1

(Zhang and Megraw, 2007)

Comments

Expression of cnn^{Δ1.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} fails to rescue the maternal effect lethality of cnn^HK21/cnn^25cn1 embryos.

(Zhang and Megraw, 2007)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

cnn^{Δ1.Scer\UAS.T:Avic\GFP-EGFP}

cnn^{Δ1.UASp.EGFP}

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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