FlyBase Allele Report: Dmel\Klp61F[HMS00552]

General Information

Symbol

Dmel\Klp61F^HMS00552

Species

D. melanogaster

Name

FlyBase ID

FBal0257240

Feature type

allele

Associated gene

Dmel\Klp61F

Associated Insertion(s)

Carried in Construct

P{TRiP.HMS00552}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

(Ni et al., 2010.12.1)

Progenitor genotype

Carried in construct

P{TRiP.HMS00552}

Cytology

Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2010.12.1)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-HMS00552

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates amyotrophic lateral sclerosis type 10

modeled by Hsap\TARDBP^{M337V.UAS.Tag:MYC}

(Huber et al., 2023)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Class I neuron ddaE neurons expressing Klp61F^HMS00552 under the control of Scer\GAL4²²¹ (together with Dicer-2 for a more efficient RNAi) show a higher rate of cell body-directed growing microtubules to successfully exit a proximal branch point, but not distal or peripheral branch points.

(Feng et al., 2021)

The expression of Klp61F^HMS00552 under the control of Scer\GAL4^{VP16.mat.αTub67C} leads to highly prevalent karyosome compaction defects in late-stage oocytes, as compared to controls: karyosomes are frequently split into multiple masses, the masses are far apart and form their own spindles, which split apart and change shape around chromosomes that move apart from each other as compared to controls; the spindles are bipolar but frequently asymmetric, associated with asymmetric centromeres, but do not show obvious central spindle defects, as compared to controls; the centromeres of homologous chromosomes are also frequently misoriented.

(Radford et al., 2017)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

Klp61F^HMS00552, Scer\GAL4^{VP16.mat.αTub67C} has karyosome phenotype, enhanceable by sub^GL00583, Scer\GAL4^{VP16.mat.αTub67C}

(Radford et al., 2017)