FlyBase Allele Report: Dmel\Tbc1d8-9[HMS00722]

General Information

Symbol

Dmel\Tbc1d8-9^HMS00722

Species

D. melanogaster

Name

FlyBase ID

FBal0257405

Feature type

allele

Associated gene

Dmel\Tbc1d8-9

Associated Insertion(s)

Carried in Construct

P{TRiP.HMS00722}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

(Ni et al., 2010.12.1)

Progenitor genotype

Carried in construct

P{TRiP.HMS00722}

Cytology

Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2010.12.1)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-HMS00722

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of nephrotic syndrome type 20

CEA

(Kampf et al., 2019)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

nephrocyte, with Scer\GAL4^pros.PMG

(Kampf et al., 2019)

nephrocyte, with Scer\GAL4^Ugt36A1.PK

(Milosavljevic et al., 2022)

slit diaphragm, with Scer\GAL4^pros.PMG

(Kampf et al., 2019)

Detailed Description

Statement

Reference

Expression of Tbc1d8-9^HMS00722 under the control of Scer\GAL4^pros.PMG leads to impaired nephrocyte function (decreased uptake of FITC-albumin), associated with slit diagram localization defects and the formation of additional, ectopic slit diaphragms deeper within the membrane invaginations.

(Kampf et al., 2019)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions