FlyBase Allele Report: Dmel\Grasp65[HMS01093]

General Information

Symbol

Dmel\Grasp65^HMS01093

Species

D. melanogaster

Name

FlyBase ID

FBal0257757

Feature type

allele

Associated gene

Dmel\Grasp65

Associated Insertion(s)

Carried in Construct

P{TRiP.HMS01093}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

(Ni et al., 2010.12.1)

Progenitor genotype

Carried in construct

P{TRiP.HMS01093}

Cytology

Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2010.12.1)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-HMS01093

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

embryonic/larval fat body | third instar larval stage, with Scer\GAL4^{SPARC-MI00329-GAL4}

(Ke et al., 2018)

extracellular matrix | third instar larval stage, with Scer\GAL4^{SPARC-MI00329-GAL4}

(Ke et al., 2018)

Detailed Description

Statement

Reference

The expression of Grasp65^HMS01093 under the control of Scer\GAL4^{SPARC-MI00329-GAL4} (and Dicer-2, for a more efficient RNAi) in the larval fat body induces defective secretion, including intracellular matrix material, as shown by the retention of both secr.GFP and Vkg.GFP.

(Ke et al., 2018)

Expression of Grasp65^HMS01093 under the control of Scer\GAL4^en-e16E does not lead to a significant difference in adult wing size nor any defects in adhesion between dorsal and ventral wing epithelia, as compared to controls.

(Ibar and Glavic, 2017)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Grasp65^HMS01093, Scer\GAL4^en-e16E is an enhancer of visible | adult stage phenotype of Scer\GAL4^en-e16E, p115^KK100066

(Ibar and Glavic, 2017)

Grasp65^HMS01093, Scer\GAL4^en-e16E is an enhancer of abnormal size | adult stage phenotype of Scer\GAL4^en-e16E, p115^KK100066

(Ibar and Glavic, 2017)

Phenotype Manifest In

Enhancer of

Statement

Reference

Grasp65^HMS01093, Scer\GAL4^en-e16E is an enhancer of wing posterior compartment | adult stage phenotype of Scer\GAL4^en-e16E, p115^KK100066

(Ibar and Glavic, 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The wing posterior compartment size reduction seen in flies expressing p115^KK100066 under the control of Scer\GAL4^en-e16E is enhanced when Grasp65^HMS01093 is co-expressed.

(Ibar and Glavic, 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

34082