FlyBase Allele Report: Dmel\Nf1[KK101909]

General Information

Symbol

Dmel\Nf1^KK101909

Species

D. melanogaster

Name

FlyBase ID

FBal0259039

Feature type

allele

Associated gene

Dmel\Nf1

Associated Insertion(s)

Carried in Construct

P{KK101909}

Also Known As

UAS-Nf1 RNAi, Nf1 RNAi, UAS-Nf1^RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

(Keleman et al., 2009.8.5)

Progenitor genotype

Carried in construct

P{KK101909}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Keleman et al., 2009.8.5)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-KK101909

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of attention deficit hyperactivity disorder

CEA

(King et al., 2016, van der Voet et al., 2016)

model of sleep disorder

CEA

(King et al., 2016)

model of neurofibromatosis

CEA

(Botero et al., 2021, King et al., 2020)

model of neurofibromatosis 1

CEA

(Durkin et al., 2023)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor rhythm, with Scer\GAL4^elav.PU

(van der Voet et al., 2016)

abnormal sleep, with Scer\GAL4^elav.PU

(van der Voet et al., 2016)

abnormal sleep | adult stage, with Scer\GAL4^30Y

(Machado Almeida et al., 2021)

abnormal sleep | adult stage, with Scer\GAL4¹⁴⁷¹

(Machado Almeida et al., 2021)

abnormal sleep | adult stage, with Scer\GAL4^c739

(Machado Almeida et al., 2021)

abnormal sleep | adult stage, with Scer\GAL4^c747

(Machado Almeida et al., 2021)

abnormal sleep | adult stage, with Scer\GAL4^Mef2.247

(Machado Almeida et al., 2021)

abnormal sleep | adult stage, with Scer\GAL4^nSyb.PU

(King et al., 2016)

abnormal sleep | male, with Scer\GAL4^elav-C155

(Maurer et al., 2020)

abnormal sleep | male, with Scer\GAL4^Rdl.PS

(Maurer et al., 2020)

abnormal sleep | male | RU486 conditional, with Scer\GAL4^{elav.Switch.PO}

(Maurer et al., 2020)

decreased sleep | larval stage, with Scer\GAL4^elav.PU

(Durkin et al., 2023)

decreased sleep | male, with Scer\GAL4^elav-C155

(Maurer et al., 2020)

decreased sleep | male | RU486 conditional, with Scer\GAL4^{elav.Switch.PO}

(Maurer et al., 2020)

increased rate of movement, with Scer\GAL4^elav.PU

(van der Voet et al., 2016)

increased rate of movement | adult stage, with Scer\GAL4^ey-OK107

(Machado Almeida et al., 2021)

increased rate of movement | adult stage, with Scer\GAL4^nSyb.PU

(King et al., 2016)

increased sleep | adult stage, with Scer\GAL4^ey-OK107

(Machado Almeida et al., 2021)

increased sleep | adult stage, with Scer\GAL4^{Mef2.247.Switch}

(Machado Almeida et al., 2021)

increased sleep | adult stage, with Scer\GAL4^Tab2-201Y

(Machado Almeida et al., 2021)

Phenotype Manifest In

adult mushroom body, with Scer\GAL4^30Y

(Machado Almeida et al., 2021)

Detailed Description

Statement

Reference

Males expressing Nf1^KK101909 under the control of Scer\GAL4^elav-C155 show decreased night-time sleep; there are more but shorter day-time sleep bouts; they recover lost sleep upon sleep deprivation although less that controls.

Expression under the control of Scer\GAL4^Rdl.PS leads to more but shorter night-time sleep bouts.

Adulthood-only expression under the control of Scer\GAL4^{elav.Switch.PO} (induced by RU486 treatment) leads to abnormal sleep - decreased night-time sleep time, more but shorter night-time sleep bouts and more day-time sleep bouts.

(Maurer et al., 2020)

Expression of Nf1^KK101909 RNAi (and UAS-Dicer2 to enhance RNAi efficiency) under the control of Scer\GAL4^nSyb.PU results in significant loss of sleep in adults both during the day and at night compared to controls. The flies display relatively sparse but large bursts of activity that are more frequent than in controls.

(King et al., 2016)

Expression of Nf1^KK101909 driven by Scer\GAL4^elav.PU results in arrhythmicity in around 70% of flies, and significant hyperactivity and sleep loss (particularly at night), compared to controls.

(van der Voet et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Nf1^KK101909, Scer\GAL4^elav-C155 has decreased sleep | male phenotype, enhanceable by Lztr1^GD4339, Scer\GAL4^elav-C155

(Maurer et al., 2020)

Nf1^KK101909, Scer\GAL4^elav-C155 has abnormal sleep | male phenotype, enhanceable by Lztr1^GD4339, Scer\GAL4^elav-C155