Expression of Dph5^KK107945 under the control of Scer\GAL4^esg-NP5130 combined with Gal80[ts] to bypass the developmental stage and Scer\GAL80^Su(H).GBE to limit the expression to intestinal stem cells (ISCs) has no significant effect on the number of ISCs, but it significantly reduced the number of actively proliferating (EdU+) cells in the posterior adult midgut Similar reduction is also observed without the addition of Scer\GAL80^Su(H).GBE) and the knock-down of Dph5 (with Scer\GAL4^esg-NP5130 and Gal80[ts]) also prevents the increase in cell proliferation in the posterior midgut epithelium under refeeding conditions (starvation followed by 24h or refeeding).

Expression of Dph5^KK107945 under Scer\GAL4^GMR11F02 does not affect the number of mitotic (pH3+) cells in the third instar larval wing disc. Scer\GAL4^nos.PG-driven expression (with UAS-Dicer2 to enhance RNAi efficacy) does not induce any loss of germline stem cells in female germaria, compared to controls.