FlyBase Allele Report: Dmel\ced-6[RNAi.UAS.cAa]

General Information

Symbol

Dmel\ced-6^RNAi.UAS.cAa

Species

D. melanogaster

Name

FlyBase ID

FBal0260810

Feature type

allele

Associated gene

Dmel\ced-6

Associated Insertion(s)

Carried in Construct

P{UAS-ced-6.RNAi.A}

Key Links

Transgenic product class

RNAi_reagent

(Awasaki et al., 2006)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Awasaki et al., 2006)

Mutagen

in vitro construct

(Awasaki et al., 2006)

Progenitor genotype

Carried in construct

P{UAS-ced-6.RNAi.A}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat of a 490bp ced-6 cDNA fragment (nucleotide positions 132-622).

(Awasaki et al., 2006)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

ced-6

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | P-stage, with Scer\GAL4^repo

(Awasaki et al., 2006)

Phenotype Manifest In

adult ensheathing glial cell, with Scer\GAL4^repo.PU

(Doherty et al., 2009)

glial cell, with Scer\GAL4^repo

(Awasaki et al., 2006)

Detailed Description

Statement

Reference

Expression of ced-6^{dsRNA.Scer\UAS.cAa} under the control of Scer\GAL4^repo.PU strongly suppresses glial clearance of axonal debris from the central nervous system after severing of olfactory receptor neurons. Expression of ced-6^{dsRNA.Scer\UAS.cAa} under the control of either Scer\GAL4^Mz709 or Scer\GAL4^alrm.PD has no effect on axon debris clearance after severing of olfactory receptor neurons.

(Doherty et al., 2009)

At 6 and 18 hours APF, the volume of the distal tip region of the mushroom body dorsal lobes which is infiltrated by glial processes is decreased in animals expressing ced-6^{dsRNA.Scer\UAS.cAa} under the control of Scer\GAL4^repo compared to wild type. The number of glial lumps over 2 μM per mushroom body lobe is severely reduced in the mutants compared to wild type at these timepoints (these lumps are normally formed by glial processes that engulf clusters of varicosities on the axon branches).

(Awasaki et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^repo, ced-6^RNAi.UAS.cAa has abnormal neuroanatomy | P-stage phenotype, enhanceable by drpr^Δ5/drpr[+]

(Awasaki et al., 2006)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^repo, ced-6^RNAi.UAS.cAa has glial cell phenotype, enhanceable by drpr^Δ5/drpr[+]

(Awasaki et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

The reduced glial infiltration phenotype and reduction in glial lumps over 2 μM per mushroom body lobe seen in animals expressing ced-6^{dsRNA.Scer\UAS.cAa} under the control of Scer\GAL4^repo at 18 hours after puparium formation is enhanced by drpr^Δ5.

(Awasaki et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

ced-6^RNAi.UAS.cAa

ced-6^{dsRNA.Scer\UAS.cAa}

ced-6^{dsRNA.UAS.cAa}

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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