FlyBase Allele Report: Hsap\SNCAIP[UAS.cHa]

General Information

Symbol

Hsap\SNCAIP^UAS.cHa

Species

H. sapiens

Name

Saccharomyces cerevisiae UAS construct of Hernandez-Vargas

FlyBase ID

FBal0260868

Feature type

allele

Associated gene

Hsap\SNCAIP

Associated Insertion(s)

Carried in Construct

P{UAS-sinf.H}

Key Links

Transgenic product class

wild_type

(Hernández-Vargas et al., 2011)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Hernández-Vargas et al., 2011)

Mutagen

in vitro construct

(Hernández-Vargas et al., 2011)

Progenitor genotype

Carried in construct

P{UAS-sinf.H}

Cytology

Description

UAS regulatory sequences drive expression of Hsap\SNCAIP.

(Hernández-Vargas et al., 2011)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Hsap\SNCAIP

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of Parkinson's disease

CEA

(Hernández-Vargas et al., 2011)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Parkinson's disease

modeled by Hsap\SNCA^A30P.UAS

(Hernández-Vargas et al., 2011)

modeled by Hsap\SNCA^A53T.UAS

(Hernández-Vargas et al., 2011)

modeled by Hsap\SNCA^UAS.cFa

(Hernández-Vargas et al., 2011)

model of Parkinson's disease

is ameliorated by Hsap\SNCA^UAS.cFa

(Hernández-Vargas et al., 2011)

is ameliorated by Hsap\SNCA^A53T.UAS

(Hernández-Vargas et al., 2011)

is ameliorated by Hsap\SNCA^A30P.UAS

(Hernández-Vargas et al., 2011)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior, with Scer\GAL4^unspecified

(Hernández-Vargas et al., 2011)

abnormal neuroanatomy, with Scer\GAL4^elav-C155

(Hernández-Vargas et al., 2011)

abnormal neuroanatomy, with Scer\GAL4^ple.PF

(Hernández-Vargas et al., 2011)

abnormal neurophysiology, with Scer\GAL4^unspecified

(Hernández-Vargas et al., 2011)

short lived, with Scer\GAL4^unspecified

(Hernández-Vargas et al., 2011)

Phenotype Manifest In

dopaminergic neuron, with Scer\GAL4^ple.PF

(Hernández-Vargas et al., 2011)

ommatidium, with Scer\GAL4^elav-C155

(Hernández-Vargas et al., 2011)

Detailed Description

Statement

Reference

Expression of Hsap\SNCAIP^Scer\UAS.cHa under the control of Scer\GAL4^elav-C155 leads to toxicity and ultrastructural abnormalities in ommatidia.

Expression of Hsap\SNCAIP^Scer\UAS.cHa under the control of Scer\GAL4^ple.PF causes a significant reduction in the number of dopaminergic neurons.

Expression of Hsap\SNCAIP^Scer\UAS.cHa under the control of Scer\GAL4^unspecified causes decreased motility and premature death.

Flies over-expressing Hsap\SNCAIP^Scer\UAS.cHa under the control of Scer\GAL4^unspecified display abnormalities in their electroretinograms (ERG). The ERGs have significantly augmented on- and off-transients. The amplitude of the sustained component of the photoreceptor potential (PP) and the overshoot (OS) are also affected. Treatment with L-Dopa/carbidopa reverts the effect of the over-expression of the transgene.

(Hernández-Vargas et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Hsap\SNCAIP^UAS.cHa, Scer\GAL4^ple.PF is a suppressor of abnormal neuroanatomy phenotype of Hsap\SNCA^UAS.cFa, Scer\GAL4^ple.PF

(Hernández-Vargas et al., 2011)

Hsap\SNCAIP^UAS.cHa, Scer\GAL4^unspecified is a suppressor of short lived phenotype of Hsap\SNCA^UAS.cFa, Scer\GAL4^unspecified

(Hernández-Vargas et al., 2011)

Hsap\SNCAIP^UAS.cHa, Scer\GAL4^unspecified is a suppressor of abnormal locomotor behavior phenotype of Hsap\SNCA^UAS.cFa, Scer\GAL4^unspecified

(Hernández-Vargas et al., 2011)

Hsap\SNCAIP^UAS.cHa, Scer\GAL4^elav-C155 is a suppressor of abnormal neuroanatomy phenotype of Hsap\SNCA^UAS.cFa, Scer\GAL4^elav-C155

(Hernández-Vargas et al., 2011)

Phenotype Manifest In

Suppressor of

Statement

Reference

Hsap\SNCAIP^UAS.cHa, Scer\GAL4^ple.PF is a suppressor of dopaminergic neuron phenotype of Hsap\SNCA^UAS.cFa, Scer\GAL4^ple.PF

(Hernández-Vargas et al., 2011)

Hsap\SNCAIP^UAS.cHa, Scer\GAL4^elav-C155 is a suppressor of ommatidium phenotype of Hsap\SNCA^UAS.cFa, Scer\GAL4^elav-C155

(Hernández-Vargas et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Co-expression of Hsap\SNCAIP^Scer\UAS.cHa and Hsap\SNCA^Scer\UAS.cFa under the control of Scer\GAL4^elav-C155 suppresses the ommatidium phenotype resulting from the expression of either transgene alone.

Co-expression of Hsap\SNCAIP^Scer\UAS.cHa and Hsap\SNCA^Scer\UAS.cFa under the control of Scer\GAL4^ple.PF increases the number of dopaminergic neurons compared with wild-type or the expression-phenotype of either transgene alone.

Flies co-expressing Hsap\SNCAIP^Scer\UAS.cHa and Hsap\SNCA^Scer\UAS.cFa via Scer\GAL4^unspecified show reduction in age-associated motility and mortality very similar to that of wild-type controls.

(Hernández-Vargas et al., 2011)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Hsap\SNCAIP^Scer\UAS.cHa

Hsap\SNCAIP^UAS.cHa

Name Synonyms

Saccharomyces cerevisiae UAS construct of Hernandez-Vargas

Secondary FlyBase IDs

References (2)

Report Sections

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