FlyBase Allele Report: Dmel\Cul4[L2-1]

General Information

Symbol

Dmel\Cul4^L2-1

Species

D. melanogaster

Name

FlyBase ID

FBal0261053

Feature type

allele

Associated gene

Dmel\Cul4

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Lin et al., 2009)

Progenitor genotype

P{EP}GE17790

(Lin et al., 2009)

Cytology

Description

Imprecise excision of P{EP}GE17790 generates an amino-terminal deletion of 396 amino acids in Cul4.

(Lin et al., 2009)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell shape | somatic clone

(Lin et al., 2009)

lethal - all die before end of second instar larval stage

(Lin et al., 2009)

some die during second instar larval stage

(Lin et al., 2009)

Phenotype Manifest In

Detailed Description

Statement

Reference

Homozygous Cul-4^L2-1 mutants are arrested in the second-instar larval stage.

Cul-4^L2-1 MARCM clones are smaller in size than wild-type twin clones. Cells within mutant cells display a rounded and shrunken morphology.

(Lin et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Cul4^L2-1 has abnormal cell shape | somatic clone phenotype, suppressible by Scer\GAL4^Tub.PU/BacA\p35^UAS.cHa

(Lin et al., 2009)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Normal cell morphology is restored in Cul-4^L2-1 MARCM wing disc cell clones that are also expressing BacA\p35^Scer\UAS.cHa with the driver Scer\GAL4^tub.PU. however, the MARCM clones remain small, with fewer cells than in wild-type twin clones, indicating that cell proliferation is affected by the lack of Cul-4 activity.

(Lin et al., 2009)

Complementation and Rescue Data

Fails to complement

Cul4^JJ11

(Lin et al., 2009)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Cul-4^L2-1

Cul4^L2-1

(Lin et al., 2009)

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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