FlyBase Allele Report: Dmel\Pp2A-29B[GD17607]

General Information

Symbol

Dmel\Pp2A-29B^GD17607

Species

D. melanogaster

Name

FlyBase ID

FBal0261065

Feature type

allele

Associated gene

Dmel\Pp2A-29B

Associated Insertion(s)

Carried in Construct

P{GD17607}

Also Known As

UAS-Pp2A-29B RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dietzl et al., 2007)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dietzl et al., 2007)

Mutagen

in vitro construct

(Dietzl et al., 2007)

Progenitor genotype

Carried in construct

P{GD17607}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dietzl et al., 2007)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD17607

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates Parkinson's disease 8

modeled by Hsap\LRRK2^{G2019S.UAS.Tag:MYC}

(Sim et al., 2020)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

ganglion mother cell | larval stage, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

gonad | male | third instar larval stage, with Scer\GAL4^C587

(La Marca et al., 2019)

larval neuroblast | larval stage, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

neuroblast | increased number, with Scer\GAL4^insc-Mz1407

(Wang et al., 2009)

neuromuscular junction, with Scer\GAL4^elav-C155

(Bulat et al., 2014)

synapse, with Scer\GAL4^elav-C155

(Bulat et al., 2014)

Detailed Description

Statement

Reference

Expressing Pp2A-29B^GD17607 under the control of Scer\GAL4^C587 (together with Dicer-2, for a more efficient RNAi) leads to early germline cell loss in L3 male gonads.

(La Marca et al., 2019)

Presynaptic expression of Pp2A-29B^GD17607 under the control of Scer\GAL4^elav-C155 causes synaptic retractions in just under 50% of synapses.

(Bulat et al., 2014)

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in loss of neuroblasts in the larval brain without any change in size of the remaining neuroblasts.

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in shorter neuroblast lineages (less daughter cells per neuroblast) in the larval brain compared to controls.

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in mild defects in the shape of neuroblasts in the larval brain.

(Neumüller et al., 2011)

Expression of Pp2A-29B^GD17607 under the control of Scer\GAL4¹⁴⁰⁷ results in neuroblast overproliferation in larval brains.

(Wang et al., 2009)

External Data

Linkouts

Neuroblasts Screen Database (Knoblich Lab) - A database for RNAi phenotypes in larval neural stem cells